Expression analysis and tissue distribution of two 14-3-3 proteins in silkworm (Bombyx mori)
文献类型：期刊论文
| 作者 | Kong, LY; Lv, ZB; Chen, J; Nie, ZM; Wang, D; Shen, HD; Wang, XD; Wu, XF; Zhang, YZ |
| 刊名 | BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
 |
| 出版日期 | 2007 |
| 卷号 | 1770期号:12页码:1598-1604 |
| 关键词 | Bombyx mori 14-3-3 protein expression analysis EST real time PCR |
| 通讯作者 | Zhang, YZ (reprint author), Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou 310018, Peoples R China.,yaozhou@chinagene.com |
| 英文摘要 | 14-3-3 proteins, which have been identified in a wide variety of eukaryotes, are highly conserved acidic proteins. In this study, we identified two genes in silkworm that encode 14-3-3 proteins (Bm14-3-3 zeta and Bm14-3-3 epsilon). Category of two 14-3-3 proteins was identified according to phylogenetic analysis. Bm14-3-3 zeta shared 90% identity with that in Drosophila, while Bm14-3-3 epsilon: shared 86% identity with that in Drosophila. According to Western blot and real time PCR analysis, the Bm14-3-3 zeta expression levels are higher than Bm14-3-3 epsilon in seven tissues and in four silkworm developmental stages examined. Bm14-3-3 zeta was expressed during every stage of silkworm and in every tissue of the fifth instar larvae that was examined, but Bm14-3-3 epsilon expression was not detected in eggs or heads of the fifth instar larvae. Both 14-3-3 proteins were highly expressed in silk glands. These results suggest that Bm14-3-3 zeta expression is universal and continuous, while Bm14-3-3 epsilon expression is tissue and stage-specific. Based on tissue expression patterns and the known functions of 14-3-3 proteins, it may be that both 14-3-3 proteins are involved in the regulation of gene expression in silkworm silk glands. (C) 2007 Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | 14-3-3 PROTEINS ; GENE-EXPRESSION ; PATTERNS ; CANCER ; ESTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000251120100002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1619]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Kong, LY,Lv, ZB,Chen, J,et al. Expression analysis and tissue distribution of two 14-3-3 proteins in silkworm (Bombyx mori)[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,2007,1770(12):1598-1604. |
| APA | Kong, LY.,Lv, ZB.,Chen, J.,Nie, ZM.,Wang, D.,...&Zhang, YZ.(2007).Expression analysis and tissue distribution of two 14-3-3 proteins in silkworm (Bombyx mori).BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,1770(12),1598-1604. |
| MLA | Kong, LY,et al."Expression analysis and tissue distribution of two 14-3-3 proteins in silkworm (Bombyx mori)".BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1770.12(2007):1598-1604. |
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