Molecular cloning, expression and characterization of protein disulfide isomerase from Conus marmoreus
文献类型：期刊论文
| 作者 | Wang, ZQ; Han, YH; Shao, XX; Chi, CW; Guo, ZY |
| 刊名 | FEBS JOURNAL
 |
| 出版日期 | 2007 |
| 卷号 | 274期号:18页码:4778-4787 |
| 关键词 | conotoxin disulfide isomerization oxidative folding protein disulfide isomerase |
| 通讯作者 | Chi, CW (reprint author), Tongji Univ, Inst Prot Res, 1239 Siping Rd, Shanghai 200092, Peoples R China.,chi@sunm.shcnc.ac.cn ; zhan-yun.guo@mail.tongji.edu.cn |
| 英文摘要 | The oxidative folding of disulfide-rich conotoxins is essential for their biological functions. In vivo, disulfide bond formation is mainly catalyzed by protein disulfide isomerase. To elucidate the physiologic roles of protein disulfide isomerase in the folding of conotoxins, we have cloned a novel full-length protein disulfide isomerase from Conus marmoreus. Its ORF encodes a 500 amino acid protein that shares sequence homology with protein disulfide isomerases from other species, and 70% homology with human protein disulfide isomerase. Enzymatic analyses of recombinant C. marmoreus protein disulfide isomerase showed that it shared functional similarities with human protein disulfide isomerase. Using conotoxins tx3a and sTx3.1 as substrate, we analyzed the oxidase and isomerase activities of the C. marmoreus protein disulfide isomerase and found that it was much more efficient than glutathione in catalyzing oxidative folding and disulfide isomerization of conotoxins. We further demonstrated that macromolecular crowding had little effect on the protein disulfide isomerase-catalyzed oxidative folding and disulfide isomerization of conotoxins. On the basis of these data, we propose that the C. marmoreus protein disulfide isomerase plays a key role during in vivo folding of conotoxins. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | CONOTOXINS ; CATALYSIS ; SUPERFAMILY ; DEPENDENCE ; PEPTIDES ; HIRUDIN ; VENOMS ; DOMAIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000249164800012 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1627]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, ZQ,Han, YH,Shao, XX,et al. Molecular cloning, expression and characterization of protein disulfide isomerase from Conus marmoreus[J]. FEBS JOURNAL,2007,274(18):4778-4787. |
| APA | Wang, ZQ,Han, YH,Shao, XX,Chi, CW,&Guo, ZY.(2007).Molecular cloning, expression and characterization of protein disulfide isomerase from Conus marmoreus.FEBS JOURNAL,274(18),4778-4787. |
| MLA | Wang, ZQ,et al."Molecular cloning, expression and characterization of protein disulfide isomerase from Conus marmoreus".FEBS JOURNAL 274.18(2007):4778-4787. |
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