Polycomb protein cbx4 promotes SUMO modification of de novo DNA methyltransferase dnmt3a
文献类型：期刊论文
| 作者 | Li, B; Zhou, J; Liu, P; Hu, JL; Jin, H; Shimono, Y; Takahashi, M; Xu, GL; Li, B; Zhou, J |
| 刊名 | BIOCHEMICAL JOURNAL
 |
| 出版日期 | 2007 |
| 卷号 | 405期号:1页码:369-378 |
| 关键词 | chromobox 4 (Cbx4) DNA methyltransferase 3a (Dnmt3a) E3 ligase small ubiquitin-related modifier (SUMO) SUMOylation Pro-Trp-Trp-Pro domain (PWWP domain) |
| 通讯作者 | Xu, GL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,glxu@sibs.ac.cn |
| 英文摘要 | The 'de novo methyltransferase' Dnmt3a (DNA methyltransferase 3a) has been shown to mediate transcriptional repression. Posttranslational modification of Dnmt3a by SUMOylation affects its ability to transcriptionally repress. However, very little is known about how the SUMOylation process is regulated. In the present study, we identified a PcG (Polycomb group) protein, Cbx4 (chromobox 4), as a specific interaction partner of Dnmt3a. Co-expression of Cbx4 and SUMO-1 (small ubiquitin-related modifier-1) along with Dnmt3a in transfected cells results in enhanced modification of Dnmt3a with SUMO-1. Purified Cbx4 also promotes SUMOylation of Dnmt3a in vitro. The modification occurs in the N-terminal regulatory region, including the PWWP (Pro-Trp-Trp-Pro) domain. Our results suggest that Cbx4 functions as a SUMO E3 ligase for Dnmt3a and it might be involved in the functional regulation of DNA methyltransferases by promoting their SUMO modification. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | MAJOR SATELLITE REPEATS ; E3 LIGASE ACTIVITY ; TRANSCRIPTIONAL REPRESSION ; PWWP DOMAIN ; PERICENTRIC HETEROCHROMATIN ; METHYLATION PATTERNS ; HISTONE H3 ; PC2 ; DEACETYLASES ; SUMOYLATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000248158600017 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1638]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, B,Zhou, J,Liu, P,et al. Polycomb protein cbx4 promotes SUMO modification of de novo DNA methyltransferase dnmt3a[J]. BIOCHEMICAL JOURNAL,2007,405(1):369-378. |
| APA | Li, B.,Zhou, J.,Liu, P.,Hu, JL.,Jin, H.,...&Xu, GL.(2007).Polycomb protein cbx4 promotes SUMO modification of de novo DNA methyltransferase dnmt3a.BIOCHEMICAL JOURNAL,405(1),369-378. |
| MLA | Li, B,et al."Polycomb protein cbx4 promotes SUMO modification of de novo DNA methyltransferase dnmt3a".BIOCHEMICAL JOURNAL 405.1(2007):369-378. |
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