The lower cytotoxicity of cinnamomin (a type II RIP) is due to its B-chain
文献类型：期刊论文
| 作者 | Wang, BZ; Zou, WG; Liu, WY; Liu, XY |
| 刊名 | ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2006 |
| 卷号 | 451期号:1页码:91-96 |
| 关键词 | cytotoxicity hybrid RIP ribosome-inactivating protein RNA N-glycosidase Scatchard analysis |
| 通讯作者 | Liu, WY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,wyliu@sibs.ac.cn |
| 英文摘要 | The cytotoxicity of intact cinnamomin (a type II ribosome-inactivating protein, RIP) and the RNA N-glycosidase activity of cinnamomin A-chain have been studied and compared with those of ricin. Cinnamomin A-chain exhibits a similar RNA N-glycosidase activity in inhibiting in vitro protein synthesis compared with that of ricin, whereas the cytotoxicity to BA/F3 beta cells of intact cinnamomin is markedly lower than intact ricin. In order to demonstrate that it is the B-chains of the two Rips that bear the difference in cytotoxicity, two hybrid RIPs are prepared from the purified A-/B-chains of cinnamomin and ricin by the disulfide exchange reaction. It has been found that hybrid RIP constructed from cinnamomin A-chain and ricin B-chain is more toxic to BA/F3 beta cells than the native cinnamomin, and equivalent to the native ricin. However, the cytotoxicity to BA/F3 beta cells of the hybrid RIP constructed from the ricin A-chain and cinnamomin B-chain is lower than ricin, equivalent to the native cinnamomin. Furthermore, the bound amounts of two B-chains on the cell surface are determined by the method of direct cellular ELISA and Scatchard analysis of the binding of the two B-chains indicates that cinnamomin and ricin share similar binding sites with different affinity. (c) 2006 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | RIBOSOME-INACTIVATING PROTEINS ; RICINUS-COMMUNIS AGGLUTININ ; GALACTOSE-BINDING ; CAMPHOR TREE ; A-CHAIN ; SEEDS ; CELLS ; ABRIN ; INHIBITORS ; MODECCIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000238796600011 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1643]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, BZ,Zou, WG,Liu, WY,et al. The lower cytotoxicity of cinnamomin (a type II RIP) is due to its B-chain[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,2006,451(1):91-96. |
| APA | Wang, BZ,Zou, WG,Liu, WY,&Liu, XY.(2006).The lower cytotoxicity of cinnamomin (a type II RIP) is due to its B-chain.ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,451(1),91-96. |
| MLA | Wang, BZ,et al."The lower cytotoxicity of cinnamomin (a type II RIP) is due to its B-chain".ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 451.1(2006):91-96. |
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