Regulation of the glutamate transporter EAAC1 by expression and activation of delta-opioid receptor
文献类型：期刊论文
| 作者 | Xia, P; Pei, G; Schwarz, W |
| 刊名 | EUROPEAN JOURNAL OF NEUROSCIENCE
 |
| 出版日期 | 2006 |
| 卷号 | 24期号:1页码:87-93 |
| 关键词 | EAAC1 glutamate uptake opioid receptor transport current Xenopus oocyte |
| 通讯作者 | Schwarz, W (reprint author), CAS, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Max Planck Guest Lab, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,gpei@sibs.ac.cn ; wolfgang.schwarz@mpibp-frankfurt.mpg.de |
| 英文摘要 | Glutamate transporters play an essential role in regulating temporal and spatial glutamate concentration in the synaptic cleft. The excitatory amino acid carrier 1 (EAAC1) is a neuronal-specific transporter. Opioid receptors are found to modulate the glutamatergic system in brain, but the regulatory mechanism remains to be investigated. Here we report that the delta-opioid receptor (DOR) co-expressed with EAAC1 in Xenopus oocytes, but not the mu-opioid receptor, down-regulates EAAC1 function, and that [D-Pen(2,5)]-enkephalin stimulation of DOR can counteract the down-regulation of the EAAC1-mediated uptake. Results from co-immunoprecipitation and immunofluorescence microscopy in both oocytes and rat hippocampal neurons indicate co-localization and suggest direct interaction between DOR and EAAC1. The results suggest that DOR can reduce EAAC1 function by direct protein-protein interaction and that activation of DOR releases the inhibitory interaction. We suggest that this mechanism may be important for modulation of the glutamatergic system following opioid stimulation. |
| 学科主题 | Neurosciences & Neurology |
| 类目[WOS] | Neurosciences |
| 关键词[WOS] | LONG-TERM POTENTIATION ; CORTICAL CELL-CULTURE ; HIPPOCAMPAL CA1 AREA ; PROTEIN-KINASE-C ; XENOPUS-OOCYTES ; NEUROTRANSMITTER TRANSPORTERS ; RAT HIPPOCAMPUS ; IN-VITRO ; LOCALIZATION ; CHANNELS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000239004100011 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1644]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xia, P,Pei, G,Schwarz, W. Regulation of the glutamate transporter EAAC1 by expression and activation of delta-opioid receptor[J]. EUROPEAN JOURNAL OF NEUROSCIENCE,2006,24(1):87-93. |
| APA | Xia, P,Pei, G,&Schwarz, W.(2006).Regulation of the glutamate transporter EAAC1 by expression and activation of delta-opioid receptor.EUROPEAN JOURNAL OF NEUROSCIENCE,24(1),87-93. |
| MLA | Xia, P,et al."Regulation of the glutamate transporter EAAC1 by expression and activation of delta-opioid receptor".EUROPEAN JOURNAL OF NEUROSCIENCE 24.1(2006):87-93. |
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