Activity identification of ribozyme and U1 snRNA chimeric ribozyme against TGF beta 1 in cell-free system and in hepatic stellate cells
文献类型：期刊论文
| 作者 | Song, YH; Liu, F; Tian, D; Xue, XL; Liu, NZ; Wu, XL; Lin, JS; Jin, YX |
| 刊名 | SCIENCE IN CHINA SERIES C-LIFE SCIENCES
 |
| 出版日期 | 2006 |
| 卷号 | 49期号:1页码:73-81 |
| 关键词 | RNA catalytic U1 snRNA TGF beta 1 HSC |
| 通讯作者 | Lin, JS (reprint author), Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Inst Liver Dis, Wuhan 430030, Peoples R China.,jslin@tjh.tjmu.edu.cn ; yxjin@sibs.ac.cn |
| 英文摘要 | Transforming growth factor beta 1 (TGF beta 1) is known to be intimately involved in many cellular processes. To explore the mechanism of TGF beta 1 in these processes, the non-chimeric hammerhead ribozyme and U1 snRNA chimeric ribozyme against TGF beta 1 were designed to down-regulate TGF beta 1 expression. The activity of non-chimeric ribozyme and U1 snRNA chimeric ribozyme against TGF beta 1 in vitro and in activated hepatic stellate cells (HSCs) was detected. Cleavage reactions of both ribozymes in vitro demonstrated that non-chimeric ribozyme possessed better cleavage activity in vitro than U1 snRNA chimeric ribozyme. The further study showed U1 snRNA chimeric ribozyme inhibited TGF beta 1 expression more efficiently than non-chimeric ribozyme in transfected HSC cells. So it indicates that the U1 snRNA chimeric ribozyme provides an alternative approach for the research on the precise mechanism of TGF beta 1 in many cellular processes and a potential therapeutic candidate for TGF beta 1-related diseases. |
| 学科主题 | Life Sciences & Biomedicine - Other Topics |
| 类目[WOS] | Biology |
| 关键词[WOS] | GROWTH-FACTOR-BETA ; HUMAN-IMMUNODEFICIENCY-VIRUS ; PRE-MESSENGER-RNA ; IN-VITRO ; TRANSFORMING GROWTH-FACTOR-BETA-1 ; EXPRESSION ; MECHANISMS ; FIBROSIS ; LIVER ; FIBROGENESIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000236156100009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1648]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Song, YH,Liu, F,Tian, D,et al. Activity identification of ribozyme and U1 snRNA chimeric ribozyme against TGF beta 1 in cell-free system and in hepatic stellate cells[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2006,49(1):73-81. |
| APA | Song, YH.,Liu, F.,Tian, D.,Xue, XL.,Liu, NZ.,...&Jin, YX.(2006).Activity identification of ribozyme and U1 snRNA chimeric ribozyme against TGF beta 1 in cell-free system and in hepatic stellate cells.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,49(1),73-81. |
| MLA | Song, YH,et al."Activity identification of ribozyme and U1 snRNA chimeric ribozyme against TGF beta 1 in cell-free system and in hepatic stellate cells".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 49.1(2006):73-81. |
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