Transiently expressed short hairpin RNA targeting 126 kDa protein of tobacco mosaic virus interferes with virus infection
文献类型：期刊论文
| 作者 | Zhao, MM; An, DR; Zhao, J; Huang, GH; He, ZH; Chen, JY |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2006 |
| 卷号 | 38期号:1页码:22-28 |
| 关键词 | tobacco mosaic virus 126 kDa protein RNA interference short hairpin RNA |
| 通讯作者 | An, DR (reprint author), NW Sci & Technol Univ Agr & Forestry, Coll Plant Protect, Yangling 712100, Peoples R China.,anmuhong@public.xa.sn.cn |
| 英文摘要 | RNA interference (RNAi) silences gene expression by guiding mRNA degradation in a sequence-specific fashion. Small interfering RNA (siRNA), an intermediate of the RNAi pathway, has been shown to be very effective in inhibiting virus infection in mammalian cells and cultured plant cells. Here, we report that Agrobacterium tumefaciens -mediated transient expression of short hairpin RNA (shRNA) could inhibit tobacco mosaic virus (TMV) RNA accumulation by targeting the gene encoding the replication-associated 126 kDa protein in intact plant tissue. Our results indicate that transiently expressed shRNA efficiently interfered with TMV infection. The interference observed is sequence-specific, and time- and site-dependent. Transiently expressed shRNA corresponding to the TMV 126 kDa protein gene did not inhibit cucumber mosaic virus (CMV), an unrelated tobamovirus. In order to interfere with TMV accumulation in tobacco leaves, it is essential for the shRNA constructs to be infiltrated into the same leaves as TMV inoculation. Our results support the view that RNAi opens the door for novel therapeutic procedures against virus diseases. We propose that a combination of the RNAi technique and Agrobacterium -mediated transient expression could be employed as a potent antiviral treatment in plants. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | DOUBLE-STRANDED-RNA ; MEDIATED INTERFERENCE ; MAMMALIAN-CELLS ; HIV-1 INFECTION ; SUPPRESSION ; PLANTS ; REPLICATION ; INDUCTION ; DEFENSE ; SYSTEM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000234840400004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1649]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhao, MM,An, DR,Zhao, J,et al. Transiently expressed short hairpin RNA targeting 126 kDa protein of tobacco mosaic virus interferes with virus infection[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2006,38(1):22-28. |
| APA | Zhao, MM,An, DR,Zhao, J,Huang, GH,He, ZH,&Chen, JY.(2006).Transiently expressed short hairpin RNA targeting 126 kDa protein of tobacco mosaic virus interferes with virus infection.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,38(1),22-28. |
| MLA | Zhao, MM,et al."Transiently expressed short hairpin RNA targeting 126 kDa protein of tobacco mosaic virus interferes with virus infection".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 38.1(2006):22-28. |
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