Effect of leukemia inhibitory factor on embryonic stem cell differentiation: implications for supporting neuronal differentiation
文献类型：期刊论文
| 作者 | He, Z; Li, JJ; Zhen, CH; Feng, LY; Ding, XY |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2006 |
| 卷号 | 27期号:1页码:80-90 |
| 关键词 | leukemia inhibitory factor embryonic stem cells neural fate embryoid bodies |
| 通讯作者 | Ding, XY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Lab Mol & Cell Biol, Shanghai 200031, Peoples R China.,xyding@sunm.shcnc.ac.cn |
| 英文摘要 | Aim: Leukemia inhibitory factor (LIF), a pleiotropic cytokine, has been used extensively in the maintenance of mouse embryonic stem cell pluripotency. In this current work, we examined the effect of the LIF signaling pathway in embryonic stem (ES) cell differentiation to a neural fate. Methods: In the presence of LIF (1000 U/mL), the production of neuronal cells derived from embryoid bodies (EB) was tested under various culture conditions. Inhibition of the LIF pathway was examined with specific inhibitors. The effects of cell apoptosis and proliferation on neural differentiation were examined. ES cell differentiation into three-germ layers was compared. Results: Under various culture conditions, neuronal differentiation was increased in the presence of LIF. Blocking the LIF-activated STAT3 signaling pathway with specific inhibitors abolished the neuronal differentiation of ES cells, whereas inhibition of the LIF-activated MEK signaling pathway impaired the differentiation of ES cells toward a glial fate. LIF suppressed cell apoptosis and promoted cell proliferation during ES cell differentiation. LIF inhibited the differentiation of ES cells to both mesoderm and extraembryonic endoderm fates, but enhanced the determination of neural progenitors. Conclusion: These results suggest that LIF plays a positive role during the differentiation of ES cells into neuronal cells. |
| 学科主题 | Chemistry; Pharmacology & Pharmacy |
| 类目[WOS] | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
| 关键词[WOS] | IN-VITRO DIFFERENTIATION ; SELF-RENEWAL ; NEURAL INDUCTION ; ES CELLS ; SIGNALING PATHWAY ; RETINOIC ACID ; MUSCLE CELLS ; STAT3 ; ACTIVATION ; SPECIFICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000234427200008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1650]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | He, Z,Li, JJ,Zhen, CH,et al. Effect of leukemia inhibitory factor on embryonic stem cell differentiation: implications for supporting neuronal differentiation[J]. ACTA PHARMACOLOGICA SINICA,2006,27(1):80-90. |
| APA | He, Z,Li, JJ,Zhen, CH,Feng, LY,&Ding, XY.(2006).Effect of leukemia inhibitory factor on embryonic stem cell differentiation: implications for supporting neuronal differentiation.ACTA PHARMACOLOGICA SINICA,27(1),80-90. |
| MLA | He, Z,et al."Effect of leukemia inhibitory factor on embryonic stem cell differentiation: implications for supporting neuronal differentiation".ACTA PHARMACOLOGICA SINICA 27.1(2006):80-90. |
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