A novel conotoxin from Conus striatus, mu-SIIIA, selectively blocking rat tetrodotoxin-resistant sodium channels
文献类型：期刊论文
| 作者 | Wang, CZ; Zhang, H; Jiang, H; Lu, WY; Zhao, ZQ; Chi, CW |
| 刊名 | TOXICON
 |
| 出版日期 | 2006 |
| 卷号 | 47期号:1页码:122-132 |
| 关键词 | toxin peptide TTX sodium channel blocker |
| 通讯作者 | Zhao, ZQ (reprint author), Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Key Lab Proteom, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,zqzhao@fudan.edu.cn ; chi@sunm.shcnc.ac.cn |
| 英文摘要 | mu-Conotoxin SIIIA, a novel blocker of tetrodotoxin-resistant (TTX-R) voltage-gated sodium channels (VGSCs) has been identified from the fish-hunting cone snail, Conus striatus. The deduced sequence consists of a 20-residue signal peptide, a 31-residue pro-peptide, and a 20-residue mature toxin with its N-terminal Gln cyclized and C-terminus amidated. mu-SIIIA shares the common cysteine arrangement with other mu-conotoxins. Besides, it exhibits high sequence homology with mu-SmIIIA, a toxin recently characterized from C. stercusmuscarum which potently blocks the TTX-R VGSCs in frog neurons. With whole-cell recording, mu-SIIIA potently and selectively inhibits the TTX-R VGSCs of dissociated adult rat small-diameter dorsal root ganglia (DRG) neurons with a dose- and time-dependent property and irreversibly. Homology-based modeling of mu-PIIIA, SHIA and SmIIIA implies that they share a common backbone conformation except at the N termini. The hydroxyl-proline residue only present in mu-PIIIA is absent and substituted by an Asp residue in mu-SIIIA and SmIIIA. Similarly, one crucial basic residue (Arg(12) in mu-PIIIA) is replaced by serine in the latter two toxins. Such differences might endow them with the capacity to selectively inhibit TTX-S or TTX-R VGSCs. Considering that TTX-R VGSCs predominantly expressed in DRG neurons play pivotal roles in the nociceptive information transmission and that their specific antagonists are still lacking, mu-SIIIA might provide a useful tool for functional studies of these channels, and potentially be developed as an efficient pain killer. (C) 2005 Elsevier Ltd. All rights reserved. |
| 学科主题 | Pharmacology & Pharmacy; Toxicology |
| 类目[WOS] | Pharmacology & Pharmacy ; Toxicology |
| 关键词[WOS] | ROOT GANGLION NEURONS ; TTX-RESISTANT ; MOLECULAR-MECHANISMS ; LOCAL-ANESTHETICS ; SENSORY NEURONS ; NA+ CHANNELS ; CURRENTS ; PEPTIDES ; CONOPEPTIDES ; VENOMS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000235381200014 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1662]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, CZ,Zhang, H,Jiang, H,et al. A novel conotoxin from Conus striatus, mu-SIIIA, selectively blocking rat tetrodotoxin-resistant sodium channels[J]. TOXICON,2006,47(1):122-132. |
| APA | Wang, CZ,Zhang, H,Jiang, H,Lu, WY,Zhao, ZQ,&Chi, CW.(2006).A novel conotoxin from Conus striatus, mu-SIIIA, selectively blocking rat tetrodotoxin-resistant sodium channels.TOXICON,47(1),122-132. |
| MLA | Wang, CZ,et al."A novel conotoxin from Conus striatus, mu-SIIIA, selectively blocking rat tetrodotoxin-resistant sodium channels".TOXICON 47.1(2006):122-132. |
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