Structural and functional analysis of natrin, a venom protein that targets various ion channels
文献类型：期刊论文
| 作者 | Wang, F; Li, H; Liu, MN; Song, H; Han, HM; Wang, QL; Yin, CC; Zhou, YC; Qi, Z; Shu, YY |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2006 |
| 卷号 | 351期号:2页码:443-448 |
| 关键词 | CRISP natrin crystal structure blocker ion channel |
| 通讯作者 | Jiang, T (reprint author), Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, 15 Datun Rd, Beijing 100101, Peoples R China.,x-ray@sun5.ibp.ac.cn |
| 英文摘要 | Cysteine-rich secretory proteins (CRISPs) are secreted single-chain proteins found in different sources. Natrin is a member of the CRISP family purified from the snake venom of Naja naja atra, which has been reported as a BKca channel blocker. In our study, crystals of natrin were obtained in two different crystal forms and the structure of one of them was solved at a resolution of 1.68 angstrom. Our electrophysiological experiments indicated that natrin can block the ion channel currents of the voltage-gated potassium channel Kv1.3. Docking analyses of the interaction between natrin and Kv1.3 revealed a novel interaction pattern different from the two previously reported K+ channel inhibition models termed "functional dyad" and "basic ring". These findings offered new insights into the function of natrin and how the specific interactions between CRISPs and different ion channels can be achieved. (c) 2006 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | RICH SECRETORY PROTEIN ; CRYSTAL-STRUCTURE ; CRISP FAMILY ; POTASSIUM CHANNELS ; K+ CHANNEL ; CYSTEINE-RICH-SECRETORY-PROTEIN-4 CRISP4 ; LIZARD VENOM ; SNAKE-VENOM ; TOXIN ; HELOTHERMINE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000242119200021 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1663]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, F,Li, H,Liu, MN,et al. Structural and functional analysis of natrin, a venom protein that targets various ion channels[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2006,351(2):443-448. |
| APA | Wang, F.,Li, H.,Liu, MN.,Song, H.,Han, HM.,...&Jiang, T.(2006).Structural and functional analysis of natrin, a venom protein that targets various ion channels.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,351(2),443-448. |
| MLA | Wang, F,et al."Structural and functional analysis of natrin, a venom protein that targets various ion channels".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 351.2(2006):443-448. |
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