Expression, purification and characterization of human GM-CSF using silkworm pupae (Bombyx mori) as a bioreactor
文献类型：期刊论文
| 作者 | Chen, J; Wu, XF; Zhang, YZ |
| 刊名 | JOURNAL OF BIOTECHNOLOGY
 |
| 出版日期 | 2006 |
| 卷号 | 123期号:2页码:236-247 |
| 关键词 | human granulocyte-macrophage colony-stimulating factor Bombyx mori nucleopolyhedrovirus silkworm pupae expression purification bioreactor |
| 通讯作者 | Zhang, YZ (reprint author), Zhejiang Sci Tech Univ, Inst Biochem, Hangzhou 310018, Peoples R China.,yaozhou@chinagene.com |
| 英文摘要 | To date, many recombinant proteins have been expressed in Bombyx mori cells or silkworm larvae, apart from in pupae. Silkworm pupae may be more suitable for the expression of heterologous proteins as a bioreactor. If maintained at an appropriate temperature, silkworm pupae could be inoculated with recombinant baculovirus for the expression of a protein of interest. In this study, human granulocyte-macrophage colony-stimulating factor was successfully expressed in silkworm pupae using B. mori nucleopolyhedrovirus, purified and characterized with respect to its physico-chemical properties. The target protein expressed had an apparent molecular mass of 29 kDa and an isoelectric point of 5.1. The protein was purified using three chromatographic steps with a final recovery of 10.3%. Finally, approximately 3.5 mg of the protein was obtained with a biological activity of up to 8.4 x 10(6) cfu mg(-1). The results of this study suggest that silkworm pupae represent a convenient and low-cost bioreactor for the expression of heterologous proteins. (c) 2005 Elsevier B.V. All rights reserved. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | COLONY-STIMULATING FACTOR ; HIGH-LEVEL EXPRESSION ; TRICHOPLUSIA NI CELLS ; INSECT LARVAE ; BACULOVIRUS VECTOR ; CULTURE CELLS ; ANIMAL-CELLS ; GLYCOSYLATION ; VIRUS ; YEAST |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000237881800013 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1667]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Chen, J,Wu, XF,Zhang, YZ. Expression, purification and characterization of human GM-CSF using silkworm pupae (Bombyx mori) as a bioreactor[J]. JOURNAL OF BIOTECHNOLOGY,2006,123(2):236-247. |
| APA | Chen, J,Wu, XF,&Zhang, YZ.(2006).Expression, purification and characterization of human GM-CSF using silkworm pupae (Bombyx mori) as a bioreactor.JOURNAL OF BIOTECHNOLOGY,123(2),236-247. |
| MLA | Chen, J,et al."Expression, purification and characterization of human GM-CSF using silkworm pupae (Bombyx mori) as a bioreactor".JOURNAL OF BIOTECHNOLOGY 123.2(2006):236-247. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。