Dominant expression of 85-kDa form of cortactin in colorectal cancer
文献类型：期刊论文
| 作者 | Zhang, LH; Tian, B; Diao, LR; Xiong, YY; Tian, SF; Zhang, BH; Li, WM; Ren, H; Li, Y; Ji, JF |
| 刊名 | JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
 |
| 出版日期 | 2006 |
| 卷号 | 132期号:2页码:113-120 |
| 关键词 | colorectal neoplasms cortactin gene expression cell movement |
| 通讯作者 | Ji, JF (reprint author), Peking Univ, Sch Oncol, Beijing Inst Canc Res, Beijing Canc Hosp, Beijing 100034, Peoples R China.,jiafuj@hotmail.com |
| 英文摘要 | Purpose: Cortactin is commonly expressed in several human cancers, which may alter their invasive or metastatic properties. Eighty five kilodalton form (p85) and 80-kDa form (p80) of cortactin are two separate bands in SDS-PAGE representing different conformational states. The objective of this study was to investigate cortactin expression in colorectal cancer (CRC). Experimental Design: Cortactin expression was studied in an eight paired laser capture microdissection (LCM) CRC tissues and matched non-cancerous epithelia by immunoblotting. The expression in 58 CRC and two cell lines, HCT8 and HCT116, was studied respectively by immunohistochemistry and confocal laser scanning immunofluorescence. Results: Dominant expression of p85 was identified in LCM-procured CRC tissues compared with equal intensity of p85 and p80 forms in non-cancerous tissues, while the amount of total cortactin was approximate. Immunohistochemistry analysis demonstrated that cortactin located in the cytoplasm of tumor cells and adjacent non-cancerous cells, and its expression was negatively correlated with TNM staging and lymphatic invasion status. However, the invasion fronts in 3 of 58 primary tumors and 28 of 39 available lymph node metastases were intensively stained. Further, immunofluorescence analysis showed that cortactin was distributed in cytoplasm and enriched in the front of the extending lamellipodia at adhering side of cultured cancer cells. Conclusions: Our results demonstrated the dominant expression of p85 form of cortactin in CRC for the first time. The enrichment of cortactin in the invasion front of some tumor cells and in the extending lamellipodia of cultured cancer cells suggests that cortactin may help cancer cell movement. |
| 学科主题 | Oncology |
| 类目[WOS] | Oncology |
| 关键词[WOS] | CHROMOSOME 11Q13 AMPLIFICATION ; SQUAMOUS-CELL CARCINOMA ; BREAST-CANCER ; TYROSINE PHOSPHORYLATION ; POOR-PROGNOSIS ; ACTIN ; OVEREXPRESSION ; CYTOSKELETON ; COMPLEX ; SITES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000233737400007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1671]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, LH,Tian, B,Diao, LR,et al. Dominant expression of 85-kDa form of cortactin in colorectal cancer[J]. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY,2006,132(2):113-120. |
| APA | Zhang, LH.,Tian, B.,Diao, LR.,Xiong, YY.,Tian, SF.,...&Ji, JF.(2006).Dominant expression of 85-kDa form of cortactin in colorectal cancer.JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY,132(2),113-120. |
| MLA | Zhang, LH,et al."Dominant expression of 85-kDa form of cortactin in colorectal cancer".JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY 132.2(2006):113-120. |
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