A novel strategy to identify the regulatory DNA-organized cooperations among transcription factors
文献类型：期刊论文
| 作者 | Niu, G; Huang, L; Wang, QO; Jiang, LC; Huang, M; Shen, P; Fei, J |
| 刊名 | FEBS LETTERS
 |
| 出版日期 | 2006 |
| 卷号 | 580期号:2页码:415-424 |
| 关键词 | strategy cooperation comprehensive perturbation transcription factor MIEP |
| 通讯作者 | Fei, J (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Model Organisms Res Ctr, 7 Bldg,319 Yueyang Rd, Shanghai 200031, Peoples R China.,jfei@sibs.ac.cn |
| 英文摘要 | To identify the functional contributions of cooperations among transcription factors on regulatory DNA is critical for understanding transcription activation. But so far there is a great lack of effective identifying methods. Here we describe a novel strategy, based on comprehensively perturbed experiments and a computational model, to identify the cooperations among NF-kappa B (p65), CREB, and AP-1 in transcription activation of human cytornegalovirus major IE1 promoter/enhancer (MIEP). In this strategy, functional profiles of protein-MIEP association and RNA synthesis are achieved through comprehensively perturbing the association of p65, CREB or AP-1 with MIEP and then subjected to the computational model. Consequently, the 'real' cooperations contributing to MIEP activation are found to comprise five but not seven types of potential cooperations. Thus, our research provides a facile systematic approach to identifying the DNA-organized cooperations among transcription factors and understanding transcription activation. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 关键词[WOS] | HUMAN CYTOMEGALO-VIRUS ; IN-VIVO TRANSFECTION ; IMMEDIATE-EARLY GENE ; AMP-RESPONSE ELEMENTS ; CYCLIC-AMP ; KAPPA-B ; DECOY OLIGODEOXYNUCLEOTIDES ; EARLY ENHANCER ; BINDING-SITE ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000234937400011 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1676]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Niu, G,Huang, L,Wang, QO,et al. A novel strategy to identify the regulatory DNA-organized cooperations among transcription factors[J]. FEBS LETTERS,2006,580(2):415-424. |
| APA | Niu, G.,Huang, L.,Wang, QO.,Jiang, LC.,Huang, M.,...&Fei, J.(2006).A novel strategy to identify the regulatory DNA-organized cooperations among transcription factors.FEBS LETTERS,580(2),415-424. |
| MLA | Niu, G,et al."A novel strategy to identify the regulatory DNA-organized cooperations among transcription factors".FEBS LETTERS 580.2(2006):415-424. |
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