Saccharomyces cerevisiae Est3p dimerizes in vitro and dimerization contributes to efficient telomere replication in vivo
文献类型：期刊论文
| 作者 | Yang, CP; Chen, YB; Meng, FL; Zhou, JQ |
| 刊名 | NUCLEIC ACIDS RESEARCH
 |
| 出版日期 | 2006 |
| 卷号 | 34期号:2页码:407-416 |
| 通讯作者 | Zhou, JQ (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci,Inst Biochem & Cell Biol, State Key Lab Mol Biol,Max Planck Jr Res Grp, Shanghai 200031, Peoples R China.,jqzhou@sibs.ac.cn |
| 英文摘要 | In Saccharomyces cerevisiae at least five genes, EST1, EST2, EST3, TLC1 and CDC13, are required for telomerase activity in vivo. The telomerase catalytic subunit Est2p and telomerase RNA subunit Tlc1 constitute the telomerase core enzyme. Est1p and Est3p are the other subunits of telomerase holoenzyme. In order to dissect the function of Est3p in telomere replication, we over-expressed and purified recombinant wild-type and mutant Est3 proteins. The wild-type protein, as well as the K71A, E104A and T115A mutants were able to dimerize in vitro, while the Est3p-D49A, -K68A or -D166A mutant showed reduced ability to dimerize. Mutations in Est3p that decreased dimerization also appeared to cause telomere shortening in vivo. Double point mutation of Est3p-D49A-K68A and single point mutation of Est3p-K68A showed similar telomere shortening, suggesting that the K68 residue might be more important for telomerase activity. The ectopic co-expression of K71A or T115A mutant with wild-type Est3p using centromere plasmids caused telomere shortening, while co-expression of the D49A, K68A, D86A or F103A mutants with wild-type Est3p had no effect on telomere length regulation. These data suggested that dimerization is important for Est3p function in vivo. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | HIV-1 REVERSE-TRANSCRIPTASE ; DNA-BINDING PROTEIN ; CATALYTIC SUBUNIT ; YEAST TELOMERASE ; CDC13 PROTEIN ; BUDDING YEAST ; RNA ; MAINTENANCE ; EST1P ; RECOMBINATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000235291300011 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1677]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yang, CP,Chen, YB,Meng, FL,et al. Saccharomyces cerevisiae Est3p dimerizes in vitro and dimerization contributes to efficient telomere replication in vivo[J]. NUCLEIC ACIDS RESEARCH,2006,34(2):407-416. |
| APA | Yang, CP,Chen, YB,Meng, FL,&Zhou, JQ.(2006).Saccharomyces cerevisiae Est3p dimerizes in vitro and dimerization contributes to efficient telomere replication in vivo.NUCLEIC ACIDS RESEARCH,34(2),407-416. |
| MLA | Yang, CP,et al."Saccharomyces cerevisiae Est3p dimerizes in vitro and dimerization contributes to efficient telomere replication in vivo".NUCLEIC ACIDS RESEARCH 34.2(2006):407-416. |
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