Can 29 kDa rhGM-CSF expressed by Silkworm pupae bioreactor bring into effect as active cytokine through orally administration?
文献类型：期刊论文
| 作者 | Zhang, YZ; Chen, J; Lv, ZB; Nie, ZM; Zhang, XY; Wu, XF |
| 刊名 | EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
 |
| 出版日期 | 2006 |
| 卷号 | 28期号:3页码:212-223 |
| 关键词 | expression rhGM-CSF oral administration absorbable effect BmNPV Bombyx mori pupae |
| 通讯作者 | Zhang, YZ (reprint author), Zhejiang Sci Tech Univ, Inst Biochem, Xiasha High Tech Zone,2 Rd, Hangzhou 310018, Peoples R China.,yaozhou@chinagene.com ; zhangxy@chinaids.org.cn |
| 英文摘要 | In order to study the effect of human granulocyte-macrophage colony-stimulating factor (hGM-CSF) as active cytokine through orally administration, we expressed hGM-CSF within silkworm pupae bioreactor. The purified rhGM-CSF named as BmrhGM-CSF is characterized as 29 kDa glycoprotein, and its biological activity was measured both in vitro and in vivo. We found out BmrhGM-CSF could stimulate the colony formation of human bone marrow cells in a dose-dependent manner whether which were treated with or without gamma-ray 24 h before. The ability of colony formation induced by BmrhGM-CSF is negatively correlated with gamma-ray intensity. As soon as 15 min post oral administration with BmrhGM-CSF labeled with I-125, an approximately 20 kDa protein fragment was detected within mice blood by SDS-PAGE followed by autoradiography. In blood sample of test mice, a protein was also recognized by anti-hGM-CSF antibody using ELISA. The immunohistochemical analysis showed that BmrhGM-CSF was detected within intestinal histiocyte. This indicated it might be absorbed into blood via intestinal microvillus. Pharmacokinetics analysis after orally administered BmrhGM-CSF in animal model of leucopenia including mice, Beagle dogs and macaques showed that: (1) BmrhGM-CSF promoted the CFU-S formation in mice spleen and the synthesis of DNA in bone marrow cells of mice; (2) BmrhGM-CSF induced bone marrow karyocyta granulocyte growth significantly in both macaques and Beagle dogs compared to the negative control group. On the 9th day of orally administration, the animal WBC significantly increased in a dose-dependant manner, in which neutrophilic granulocyte was predominant. The WBC level of dogs in high dose group was about 1.5 x 10(9) cells/L more than that in the negative control. And the bone marrow smear revealed that the percents of both myloblast and progranulocyte in WBC in the hGM-CSF group were obviously higher than those in the negative control. These results proved that BmrhGM-CSF, a 29 kDa glycoprotein expressed by Silkworm pupae bioreactor, could bring into the effect as active cytokine through oral administration. (c) 2006 Elsevier B.V. All rights reserved. |
| 学科主题 | Pharmacology & Pharmacy |
| 类目[WOS] | Pharmacology & Pharmacy |
| 关键词[WOS] | COLONY-STIMULATING FACTOR ; BOMBYX CYSTEINE PROTEINASE ; FACTOR HGM-CSF ; CHYMOTRYPSIN INHIBITOR ; PROPEPTIDE REGIONS ; INSECT CELLS ; HIGH-LEVEL ; GRANULOCYTE ; MORI ; CULTURE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000238459800006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1682]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, YZ,Chen, J,Lv, ZB,et al. Can 29 kDa rhGM-CSF expressed by Silkworm pupae bioreactor bring into effect as active cytokine through orally administration?[J]. EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES,2006,28(3):212-223. |
| APA | Zhang, YZ,Chen, J,Lv, ZB,Nie, ZM,Zhang, XY,&Wu, XF.(2006).Can 29 kDa rhGM-CSF expressed by Silkworm pupae bioreactor bring into effect as active cytokine through orally administration?.EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES,28(3),212-223. |
| MLA | Zhang, YZ,et al."Can 29 kDa rhGM-CSF expressed by Silkworm pupae bioreactor bring into effect as active cytokine through orally administration?".EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES 28.3(2006):212-223. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。