Analysis of the interaction between hPFTAIRE1 and PLZF in a yeast two-hybrid system
文献类型：期刊论文
| 作者 | Gao, YK; Jiang, M; Yang, T; Chen, JY |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2006 |
| 卷号 | 38期号:3页码:164-170 |
| 关键词 | hPFTAIRE1 PLZF PCTIARE1 yeast two-hybrid interaction |
| 通讯作者 | Chen, JY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,jychen@sunm.shcnc.ac.cn |
| 英文摘要 | hPFTAIRE1 is a Cdc2-related kinase family member. To search its substrates and regulatory proteins, hPFTAIRE1 was fused to LexA and used as a bait to screen a human brain LexA two-hybrid library. In this screening, seven hPFTAIRE1 interacting proteins, including promyelocytic leukemia zinc finger (PLZF), were obtained. The interaction between PLZF and hPFTAIRE1 was confirmed by beta-galactosidase assay and Leu growth activity. PLZF encodes a transcription factor belonging to the POZ/BTB domain and Kruppel zinc finger (POK) family. The highly conserved POZ/BTB domain plays a critical role in protein-protein interaction. We deleted the POZ/BTB and Kruppel zinc finger domains, respectively, and observed the interaction between hPFTAIRE1 and truncated PLZFs by liquid beta-galactosidase activity assay. A weak interaction was detected between hPFTAIRE1 and PLZF. We also observed the interaction between PLZF and another Cdc2-related kinase, PCTAIRE1. A similar result was observed. The interaction between PLZF and hPFTAIRE1 or PCTAIRE1 was confirmed by co-immunoprecipitation assay in a yeast system. PLZF is a phosphoprotein and plays multiple roles during cell growth. Our results suggest that hPFTAIRE1 and PCTAIRE1 may play important roles in the functional regulation of PLZF. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | ACUTE PROMYELOCYTIC LEUKEMIA ; ZINC-FINGER PROTEIN ; CDK FAMILY-MEMBER ; RAR-ALPHA ; KINASE-ACTIVITY ; CELL-GROWTH ; FUSION ; EXPRESSION ; IDENTIFICATION ; TRANSLOCATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000235937800003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1684]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Gao, YK,Jiang, M,Yang, T,et al. Analysis of the interaction between hPFTAIRE1 and PLZF in a yeast two-hybrid system[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2006,38(3):164-170. |
| APA | Gao, YK,Jiang, M,Yang, T,&Chen, JY.(2006).Analysis of the interaction between hPFTAIRE1 and PLZF in a yeast two-hybrid system.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,38(3),164-170. |
| MLA | Gao, YK,et al."Analysis of the interaction between hPFTAIRE1 and PLZF in a yeast two-hybrid system".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 38.3(2006):164-170. |
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