中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Recombinant functional human lactoferrin expressed in baculovirus system

文献类型:期刊论文

作者Liu, T; Zhang, YZ; Wu, XF
刊名ACTA BIOCHIMICA ET BIOPHYSICA SINICA
出版日期2006
卷号38期号:3页码:201-206
关键词bacteriostatic activity baculovirus Bombyx mori human lactoferrin reverse transcription-polymerase chain reaction
通讯作者Zhang, YZ (reprint author), Zhejiang Sci Tec Univ, Inst Biochem, Hangzhou 310018, Peoples R China.,yaozhou@chinagene.com
英文摘要Human lactoferrin (hLf) is a multifunctional iron-binding glycoprotein. In this study, we amplified hLf cDNA by reverse transcription-polymerase chain reaction from normal human mammary gland. The nucleotide sequence of the hLf was identical to the known hLf. We constructed a recombinant virus, vBm-hLf, harboring the hLf gene and exploited the BmN cells as host to produce recombinant human lactoferrin (rhLf). It was found that a recombinant protein with a molecular mass of approximately 78 kDa was expressed. Approximately 13.5 mu g rhLf was purified from 1-2 x 10(5) BmN cells infected by vBm-hLf and the rhLf proved to be biologically active. This method established in our study will pave the way for efficient production of rhLf for further application of this protein in the future.
学科主题Biochemistry & Molecular Biology; Biophysics
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]HIGH-LEVEL EXPRESSION ; BOMBYX-MORI ; SILKWORM ; LARVAE ; CELLS ; MILK ; PURIFICATION ; GROWTH ; MICE
收录类别SCI
语种英语
WOS记录号WOS:000235937800008
版本出版稿
源URL[http://202.127.25.143/handle/331003/1685]  
专题上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式
GB/T 7714
Liu, T,Zhang, YZ,Wu, XF. Recombinant functional human lactoferrin expressed in baculovirus system[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2006,38(3):201-206.
APA Liu, T,Zhang, YZ,&Wu, XF.(2006).Recombinant functional human lactoferrin expressed in baculovirus system.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,38(3),201-206.
MLA Liu, T,et al."Recombinant functional human lactoferrin expressed in baculovirus system".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 38.3(2006):201-206.

入库方式: OAI收割

来源:上海生物化学与细胞生物学研究所

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