Recombinant functional human lactoferrin expressed in baculovirus system
文献类型：期刊论文
| 作者 | Liu, T; Zhang, YZ; Wu, XF |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2006 |
| 卷号 | 38期号:3页码:201-206 |
| 关键词 | bacteriostatic activity baculovirus Bombyx mori human lactoferrin reverse transcription-polymerase chain reaction |
| 通讯作者 | Zhang, YZ (reprint author), Zhejiang Sci Tec Univ, Inst Biochem, Hangzhou 310018, Peoples R China.,yaozhou@chinagene.com |
| 英文摘要 | Human lactoferrin (hLf) is a multifunctional iron-binding glycoprotein. In this study, we amplified hLf cDNA by reverse transcription-polymerase chain reaction from normal human mammary gland. The nucleotide sequence of the hLf was identical to the known hLf. We constructed a recombinant virus, vBm-hLf, harboring the hLf gene and exploited the BmN cells as host to produce recombinant human lactoferrin (rhLf). It was found that a recombinant protein with a molecular mass of approximately 78 kDa was expressed. Approximately 13.5 mu g rhLf was purified from 1-2 x 10(5) BmN cells infected by vBm-hLf and the rhLf proved to be biologically active. This method established in our study will pave the way for efficient production of rhLf for further application of this protein in the future. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | HIGH-LEVEL EXPRESSION ; BOMBYX-MORI ; SILKWORM ; LARVAE ; CELLS ; MILK ; PURIFICATION ; GROWTH ; MICE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000235937800008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1685]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, T,Zhang, YZ,Wu, XF. Recombinant functional human lactoferrin expressed in baculovirus system[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2006,38(3):201-206. |
| APA | Liu, T,Zhang, YZ,&Wu, XF.(2006).Recombinant functional human lactoferrin expressed in baculovirus system.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,38(3),201-206. |
| MLA | Liu, T,et al."Recombinant functional human lactoferrin expressed in baculovirus system".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 38.3(2006):201-206. |
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