Proteomic analysis of the cerebrospinal fluid of patients with lumbar disk herniation
文献类型：期刊论文
| 作者 | Liu, XD; Zeng, BF; Xu, JG; Zhu, HB; Xia, QC |
| 刊名 | PROTEOMICS
 |
| 出版日期 | 2006 |
| 卷号 | 6期号:3页码:1019-1028 |
| 关键词 | 2-DE cerebrospinal fluid lumbar disk herniation MS |
| 通讯作者 | Zeng, BF (reprint author), Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 6, Dept Orthopaed Surg, 600 Yishan Rd, Shanghai 200233, Peoples R China.,bingfangzeng@yahoo.com.cn |
| 英文摘要 | To better understand the pathophysiologic mechanisms underlying spinal nerve root injury induced by lumbar disk herniation (LDH), comparative proteomic analysis of cerebrospinal fluid (CSF) between patients with LDH (the experiment group) and the otherwise healthy patients who had had implants removed from healed fractures in the lower limbs (the control group) was carried out using 2-DE followed by LC-IT-MS and database searching. Image analysis of silver-stained 2-DE gels revealed that 15 protein spots showed significant differential expression between the two groups of CSF samples (p < 0.05). After searching the database we found that in CSF of LDH patients, the expression of cystatin C, apolipoprotein A-IV, vitamin D-binding protein, neurofilament triplet L protein, IgG, tetranecfin, and hemoglobin were elevated. However, ProSAAS, prostagladin D2 synthase, creatine kinase B, superoxide dismutase I and peroxiredoxin 2 were decreased. The subsequent ELISA measured the concentration of tetranecfin, vitamin D-binding protein and cystatin C and confirmed the results of proteomic analysis. These identified proteins are involved in the pathophysiological process of spinal nerve root injury caused by herniated lumbar disk. The functional implications of the alterations in the levels of these proteins are discussed in this paper. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology |
| 关键词[WOS] | AMYOTROPHIC-LATERAL-SCLEROSIS ; AUTOLOGOUS NUCLEUS PULPOSUS ; CUZN-SUPEROXIDE DISMUTASE ; NERVE ROOT COMPRESSION ; PORCINE CAUDA-EQUINA ; SCIATIC-NERVE ; IN-VITRO ; TETRANECTIN ; DISEASE ; PROTEINS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000235414600028 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1695]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, XD,Zeng, BF,Xu, JG,et al. Proteomic analysis of the cerebrospinal fluid of patients with lumbar disk herniation[J]. PROTEOMICS,2006,6(3):1019-1028. |
| APA | Liu, XD,Zeng, BF,Xu, JG,Zhu, HB,&Xia, QC.(2006).Proteomic analysis of the cerebrospinal fluid of patients with lumbar disk herniation.PROTEOMICS,6(3),1019-1028. |
| MLA | Liu, XD,et al."Proteomic analysis of the cerebrospinal fluid of patients with lumbar disk herniation".PROTEOMICS 6.3(2006):1019-1028. |
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