Production of Alcaligenes faecalis penicillin G acylase in Bacillus subtilis WB600 (pMA5) fed with partially hydrolyzed starch
文献类型：期刊论文
| 作者 | Zhang, M; Shi, ML; Zhou, Z; Yang, S; Yuan, ZY; Ye, Q |
| 刊名 | ENZYME AND MICROBIAL TECHNOLOGY
 |
| 出版日期 | 2006 |
| 卷号 | 39期号:4页码:555-560 |
| 关键词 | penicillin G acylase Bacillus subtilis starch pH-stat fed-batch culture |
| 通讯作者 | Ye, Q (reprint author), E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China.,qye@ecust.edu.cn |
| 英文摘要 | In this study, a recombinant strain Bacillus subtilis WB600 (pMA5) was used to produce Alcaligenes faecalis penicillin G acylase (PGA). The PGA activity and overall PGA productivity were respectively 378 and 7.13 U/l in a batch culture using a complex medium containing 25 g/l of soluble starch. Fed-batch cultures indicated that glucose had a negative effect on the production of PGA. Starch is a good carbon source, but it could not be directly used as a feed due to high viscosity after heat sterilization. A feed was prepared by limited hydrolysis of starch with a small amount of alpha-amylase, which greatly improved the fluidity with little formation of glucose. The production of PGA increased to 546 U/l by feeding of the partially hydrolyzed starch. When a mixture containing the partially hydrolyzed starch and Tryptone was added by using a pH-stat strategy, the PGA activity reached 1960 U/l, over five-fold of that obtained in the batch culture, and the overall productivity reached 19.6 U/l h, which was more than two-fold of that in the batch culture. (c) 2006 Elsevier Inc. All rights reserved. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | RECOMBINANT ESCHERICHIA-COLI ; EXPRESSION ; PURIFICATION ; SECRETION ; CULTURES ; CLONING |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000238932500005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1699]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, M,Shi, ML,Zhou, Z,et al. Production of Alcaligenes faecalis penicillin G acylase in Bacillus subtilis WB600 (pMA5) fed with partially hydrolyzed starch[J]. ENZYME AND MICROBIAL TECHNOLOGY,2006,39(4):555-560. |
| APA | Zhang, M,Shi, ML,Zhou, Z,Yang, S,Yuan, ZY,&Ye, Q.(2006).Production of Alcaligenes faecalis penicillin G acylase in Bacillus subtilis WB600 (pMA5) fed with partially hydrolyzed starch.ENZYME AND MICROBIAL TECHNOLOGY,39(4),555-560. |
| MLA | Zhang, M,et al."Production of Alcaligenes faecalis penicillin G acylase in Bacillus subtilis WB600 (pMA5) fed with partially hydrolyzed starch".ENZYME AND MICROBIAL TECHNOLOGY 39.4(2006):555-560. |
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