cDNA cloning of two novel T-superfamily conotoxins from Conus leopardus
文献类型：期刊论文
| 作者 | Chen, WH; Han, YH; Wang, Q; Miao, XW; Ou, L; Shao, XX |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2006 |
| 卷号 | 38期号:4页码:287-291 |
| 关键词 | Conus leopardus T-superfamily conotoxin cDNA cloning |
| 通讯作者 | Shao, XX (reprint author), Tongji Univ, Inst Prot Res, Shanghai 200092, Peoples R China.,shxx@sibs.ac.cn |
| 英文摘要 | The full-length cDNAs of two novel T-superfamily conotoxins, Lp5.1 and Lp5.2, were cloned from a vermivorous cone snail Conus leopardus using 3'/5'-rapid amplification of cDNA ends. The cDNA of Lp5.1 encodes a precursor of 65 residues, including a 22-residue signal peptide, a 28-residue propeptide and a 15-residue mature peptide. Lp5.1 is processed at the common signal site -X-Arg-immediately before the mature peptide sequences. In the case of Lp5.2, the precursor includes a 25-residue signal peptide and a 43-residue sequence comprising the propeptide and mature peptide, which is probably cleaved to yield a 29-residue propeptide and a 14-residue mature toxin. Although these two conotoxins share a similar signal sequence and a conserved disulfide pattern with the known T-superfamily, the pro-region and mature peptides are of low identity, especially Lp5.2 with an identity as low as 10.7% compared with the reference Mr5.1a. The elucidated cDNAs of these two toxins will facilitate a better understanding of the species distribution, the sequence diversity of T-superfamily conotoxins, the special gene structure and the evolution of these peptides. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | NICOTINIC ACETYLCHOLINE-RECEPTOR ; CYSTEINE PATTERN ; ALPHA-CONOTOXINS ; VENOM PEPTIDES ; A-SUPERFAMILY ; CHANNEL ; MARMOREUS ; TEXTILE ; UNIQUE ; TOOLS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000236911900010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1702]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Chen, WH,Han, YH,Wang, Q,et al. cDNA cloning of two novel T-superfamily conotoxins from Conus leopardus[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2006,38(4):287-291. |
| APA | Chen, WH,Han, YH,Wang, Q,Miao, XW,Ou, L,&Shao, XX.(2006).cDNA cloning of two novel T-superfamily conotoxins from Conus leopardus.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,38(4),287-291. |
| MLA | Chen, WH,et al."cDNA cloning of two novel T-superfamily conotoxins from Conus leopardus".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 38.4(2006):287-291. |
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