Two novel proteins bind specifically to trichosanthin on choriocarcinoma cell membrane
文献类型：期刊论文
| 作者 | Xia, XC; Hou, FJ; Li, J; Ke, YB; Nie, HL |
| 刊名 | JOURNAL OF BIOCHEMISTRY
 |
| 出版日期 | 2006 |
| 卷号 | 139期号:4页码:725-731 |
| 关键词 | cell surface biotinylation choriocarcinoma cells cytotoxicity radioligand binding assay trichosanthin |
| 通讯作者 | Nie, HL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,hlnie@sibs.ac.cn |
| 英文摘要 | Trichosanthin is the active protein component in the Chinese herb Trichosanthes kirilowi, which has distinct pharmacological properties. The cytotoxicity of trichosanthin was demonstrated by its selective inhibition of various choriocarcinoma cells. When Jar cells were treated with trichosanthin, the influx of calcium into the cells was observed by confocal laser scanning microscopy. When the distribution of trichosanthin-binding proteins on Jar cells was studied, two classes of binding sites for trichosanthin were shown by radioligand binding assay. Furthermore, the cytoplasmic membrane of Jar cells was biotinylated and the trichosanthin-binding proteins were isolated with trichosanthin-coupled Sepharose beads. Two protein bands with molecular masses of about 50 kDa and 60 kDa were revealed, further characterization of which should shed light on the mechanism of the selective cytotoxicity of trichosanthin to Jar cells. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | LYMPHOCYTE-SURFACE ; CHEMOKINE ; RECEPTOR ; REPLICATION ; ACTIVATION ; EXPRESSION ; CHEMISTRY ; MONOCYTES ; APOPTOSIS ; INVITRO |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000237887000012 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1705]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xia, XC,Hou, FJ,Li, J,et al. Two novel proteins bind specifically to trichosanthin on choriocarcinoma cell membrane[J]. JOURNAL OF BIOCHEMISTRY,2006,139(4):725-731. |
| APA | Xia, XC,Hou, FJ,Li, J,Ke, YB,&Nie, HL.(2006).Two novel proteins bind specifically to trichosanthin on choriocarcinoma cell membrane.JOURNAL OF BIOCHEMISTRY,139(4),725-731. |
| MLA | Xia, XC,et al."Two novel proteins bind specifically to trichosanthin on choriocarcinoma cell membrane".JOURNAL OF BIOCHEMISTRY 139.4(2006):725-731. |
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