In vivo analysis of fibroin heavy chain signal peptide of silkworm Bombyx mori using recombinant baculovirus as vector
文献类型：期刊论文
| 作者 | Wang, SP; Guo, TQ; Guo, XY; Huang, JT; Lu, CD |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2006 |
| 卷号 | 341期号:4页码:1203-1210 |
| 关键词 | signal peptide fibroin heavy chain silkworm Bombyx mori recombinant AcMNPV his-tag |
| 通讯作者 | Lu, CD (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai, Peoples R China.,cdlu@sibs.ac.cn |
| 英文摘要 | In order to investigate the functional signal peptide of silkworm fibroin heavy chain (FibH) and the effect of N- and C-terminal parts of FibH on the secretion of FibH in vivo, N- and C-terminal segments of fibh gene were fused with enhanced green fluorescent protein (EGFP) gene. The fused gene was then introduced into silkworm larvae and expressed in silk gland using recombinant AcMNPV (Autographa californica multiple nuclear polyhedrosis virus) as vector. The fluorescence of EGFP was observed with fluorescence microscope. FibH-EGFP fusion proteins extracted from silk gland were analyzed by Western blot. Results showed that the two alpha helices within N-terminal 163 amino acid residues and the C-terminal 61 amino acid residues were not necessary for cleavage of signal peptide and secretion of the fusion protein into silk gland. Then the C-terminal 61 amino acid residues were substituted with a His-tag in the fusion protein to facilitate the purification. N-terminal sequencing of the purified protein showed that the signal cleavage site is between position 21 and 22 amino acid residues. (c) 2006 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | LINKED OLIGOSACCHARIDE CHAINS ; AMINO-ACID-SEQUENCE ; TRANSGENIC SILKWORMS ; SUBUNIT COMBINATION ; ELEMENTARY UNIT ; CLEAVAGE SITES ; GENE ; SECRETION ; LIGHT ; PROTEINS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000235699900043 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1711]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, SP,Guo, TQ,Guo, XY,et al. In vivo analysis of fibroin heavy chain signal peptide of silkworm Bombyx mori using recombinant baculovirus as vector[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2006,341(4):1203-1210. |
| APA | Wang, SP,Guo, TQ,Guo, XY,Huang, JT,&Lu, CD.(2006).In vivo analysis of fibroin heavy chain signal peptide of silkworm Bombyx mori using recombinant baculovirus as vector.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,341(4),1203-1210. |
| MLA | Wang, SP,et al."In vivo analysis of fibroin heavy chain signal peptide of silkworm Bombyx mori using recombinant baculovirus as vector".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 341.4(2006):1203-1210. |
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