Characterization of ATPase activity of recombinant human Pif1
文献类型:期刊论文
作者 | Huang, Y; Zhang, DH; Zhou, JQ |
刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
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出版日期 | 2006 |
卷号 | 38期号:5页码:335-341 |
关键词 | Pif1 helicase telomere telomerase ATPase |
通讯作者 | Zhou, JQ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Max Planck Jr Res Grp,State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,jqzhou@sibs.ac.cn |
英文摘要 | Saccharomyces cerevisiae Pif1p helicase is the founding member of the Pif1 subfamily that is conserved from yeast to human. The potential human homolog of the yeast PIF1 gene has been cloned from the cDNA library of the Hek293 cell line. Here, we described a purification procedure of glutathione S-transferase (GST)-fused N terminal truncated human Pif1 protein (hPif1 AN) from yeast and characterized the enzymatic kinetics of its ATP hydrolysis activity. The ATPase activity of human Pif1 is dependent on divalent cation, such as Mg2+, Ca2+ and single-stranded DNA. Km for ATP for the ATPase activity is approximately 200 mu M. AS the ATPase activity is essential for hPif1's helicase activity, these results will facilitate the further investigation on hPif1. |
学科主题 | Biochemistry & Molecular Biology; Biophysics |
类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
关键词[WOS] | REPLICATION FORK PROGRESSION ; YEAST MITOCHONDRIAL-DNA ; SACCHAROMYCES-CEREVISIAE ; HELICASE ; TELOMERASE ; PURIFICATION ; SUBFAMILY ; CLONING ; REPAIR ; CELLS |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000237608500007 |
版本 | 出版稿 |
源URL | [http://202.127.25.143/handle/331003/1718] ![]() |
专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
推荐引用方式 GB/T 7714 | Huang, Y,Zhang, DH,Zhou, JQ. Characterization of ATPase activity of recombinant human Pif1[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2006,38(5):335-341. |
APA | Huang, Y,Zhang, DH,&Zhou, JQ.(2006).Characterization of ATPase activity of recombinant human Pif1.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,38(5),335-341. |
MLA | Huang, Y,et al."Characterization of ATPase activity of recombinant human Pif1".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 38.5(2006):335-341. |
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