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Characterization of ATPase activity of recombinant human Pif1

文献类型：期刊论文

作者Huang, Y; Zhang, DH; Zhou, JQ
刊名ACTA BIOCHIMICA ET BIOPHYSICA SINICA
出版日期2006
卷号38期号:5页码:335-341
关键词Pif1 helicase telomere telomerase ATPase
通讯作者Zhou, JQ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Max Planck Jr Res Grp,State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,jqzhou@sibs.ac.cn
英文摘要Saccharomyces cerevisiae Pif1p helicase is the founding member of the Pif1 subfamily that is conserved from yeast to human. The potential human homolog of the yeast PIF1 gene has been cloned from the cDNA library of the Hek293 cell line. Here, we described a purification procedure of glutathione S-transferase (GST)-fused N terminal truncated human Pif1 protein (hPif1 AN) from yeast and characterized the enzymatic kinetics of its ATP hydrolysis activity. The ATPase activity of human Pif1 is dependent on divalent cation, such as Mg2+, Ca2+ and single-stranded DNA. Km for ATP for the ATPase activity is approximately 200 mu M. AS the ATPase activity is essential for hPif1's helicase activity, these results will facilitate the further investigation on hPif1.
学科主题Biochemistry & Molecular Biology; Biophysics
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]REPLICATION FORK PROGRESSION ; YEAST MITOCHONDRIAL-DNA ; SACCHAROMYCES-CEREVISIAE ; HELICASE ; TELOMERASE ; PURIFICATION ; SUBFAMILY ; CLONING ; REPAIR ; CELLS
收录类别SCI
语种英语
WOS记录号WOS:000237608500007
版本出版稿
源URL[http://202.127.25.143/handle/331003/1718]  
专题上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式
GB/T 7714		 Huang, Y,Zhang, DH,Zhou, JQ. Characterization of ATPase activity of recombinant human Pif1[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2006,38(5):335-341.
APA Huang, Y,Zhang, DH,&Zhou, JQ.(2006).Characterization of ATPase activity of recombinant human Pif1.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,38(5),335-341.
MLA Huang, Y,et al."Characterization of ATPase activity of recombinant human Pif1".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 38.5(2006):335-341.

入库方式： OAI收割

来源：上海生物化学与细胞生物学研究所

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