Agkistin-s, a disintegrin domain, inhibits angiogenesis and induces BAECs apoptosis
文献类型：期刊论文
| 作者 | Ren, AX; Wang, SH; Cai, WJ; Yang, GZ; Zhu, YC; Wu, XF; Zhang, YZ |
| 刊名 | JOURNAL OF CELLULAR BIOCHEMISTRY
 |
| 出版日期 | 2006 |
| 卷号 | 99期号:6页码:1517-1523 |
| 关键词 | agkistin-s disintegrin apoptosis BAECs angiogenesis |
| 通讯作者 | Zhang, YZ (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Biochem & Cell Biol, Shanghai Inst Biol Sci,State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,yaozhou@chinagene.com |
| 英文摘要 | Previous work in our laboratory has shown agkistin, a snake venom metal loproteases (SVMPs) from the venom of Agkistrodon halys, possesses antiplatelet aggregation activity. In this study, we further examined the antiangiogenic activity of agkistin-s, the disintegrin domain of agkistin. Recombinant agkistin-s was produced in Escherichia coli by subcloning its cDNA into pET28a vector, and the effect of purified agkistin-s was evaluated. At the concentration of 0.5-1.5 mu M, the recombinant agkistin-s exhibited inhibitory activities on the bovine aortic endothelial cells (BAECs) migration and proliferation in a dose-dependent manner. In addition, it exhibited an effective antiangiogenic effect when assayed by using the 10-clay-old embryo chick CAM model and effectively inhibits the tube-like structure formation. Furthermore, it potently induced BAECs apoptosis as examined by flow cytometric assays. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | SNAKE-VENOM DISINTEGRIN ; TUMOR ANGIOGENESIS ; GROWTH-FACTOR ; ADHESION ; MODEL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000242122500005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1722]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Ren, AX,Wang, SH,Cai, WJ,et al. Agkistin-s, a disintegrin domain, inhibits angiogenesis and induces BAECs apoptosis[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2006,99(6):1517-1523. |
| APA | Ren, AX.,Wang, SH.,Cai, WJ.,Yang, GZ.,Zhu, YC.,...&Zhang, YZ.(2006).Agkistin-s, a disintegrin domain, inhibits angiogenesis and induces BAECs apoptosis.JOURNAL OF CELLULAR BIOCHEMISTRY,99(6),1517-1523. |
| MLA | Ren, AX,et al."Agkistin-s, a disintegrin domain, inhibits angiogenesis and induces BAECs apoptosis".JOURNAL OF CELLULAR BIOCHEMISTRY 99.6(2006):1517-1523. |
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