Synthesis and biological activity of seleno sunflower trypsin inhibitor analog
文献类型：期刊论文
| 作者 | Guo, X; Shi, JH; Tang, ZY; Cui, DF; Zhang, YS |
| 刊名 | CHEMICAL BIOLOGY & DRUG DESIGN
 |
| 出版日期 | 2006 |
| 卷号 | 68期号:6页码:341-344 |
| 关键词 | isosteric replacement peptide synthesis seleno sunflower trypsin inhibitor |
| 通讯作者 | Zhang, YS (reprint author), Tongji Univ, Inst Prot Res, Shanghai 200092, Peoples R China.,yszhang@sibs.ac.cn |
| 英文摘要 | Sunflower trypsin inhibitor (SFTI-1) is a cyclic peptide with 14 amino acid residues and one disulfide bond. Its synthetic acyclic analog (aSFTI-1) with N-terminal Gly and C-terminal Asp was still active. Here, we report the synthesis of seleno aSFTI-1 with the disulfide bond of aSFTI-1 replaced by diselenide bond. The formation of the diselenide bond from selenol was achieved in a single step without the aid of oxidizing agent. For comparison, aSFTI-1 itself and aSFTI-1 with its disulfide bond replaced by two serines ([Ser(3,11)] aSFTI-1) were also synthesized. The trypsin inhibitory constants of seleno aSFTI-1, aSFTI-1 and [Ser(3,11)] aSFTI-1 were determined as 6.50 x 10(-9), 1.96 x 10(-9) and 8.10 x 10(-6) respectively, indicating that the disulfide bond is essential for the structure and function of aSFTI-1, and seleno aSFTI-1 is still active, although its inhibitory constant is reduced to 30% in comparison with that of aSFTI-1. |
| 学科主题 | Biochemistry & Molecular Biology; Pharmacology & Pharmacy |
| 类目[WOS] | Biochemistry & Molecular Biology ; Chemistry, Medicinal |
| 关键词[WOS] | SEEDS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000242835300008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1723]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Guo, X,Shi, JH,Tang, ZY,et al. Synthesis and biological activity of seleno sunflower trypsin inhibitor analog[J]. CHEMICAL BIOLOGY & DRUG DESIGN,2006,68(6):341-344. |
| APA | Guo, X,Shi, JH,Tang, ZY,Cui, DF,&Zhang, YS.(2006).Synthesis and biological activity of seleno sunflower trypsin inhibitor analog.CHEMICAL BIOLOGY & DRUG DESIGN,68(6),341-344. |
| MLA | Guo, X,et al."Synthesis and biological activity of seleno sunflower trypsin inhibitor analog".CHEMICAL BIOLOGY & DRUG DESIGN 68.6(2006):341-344. |
入库方式： OAI收割
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