中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
A Cdc2-related protein kinase hPFTAIRE1 from human brain interacting with 14-3-3 proteins

文献类型:期刊论文

作者Gao, YK; Jiang, M; Yang, T; Ni, J; Chen, JY
刊名CELL RESEARCH
出版日期2006
卷号16期号:6页码:539-547
关键词hPFTAIRE1 14-3-3 proteins 14-3-3 binding motif two-hybrid
通讯作者Chen, JY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,jychen@sunm.shcnc.ac.cn
英文摘要hPFTAIRE1 (PFTK1), a Cdc2-related protein kinase, is highly expressed in human brain. It exhibits cytoplasmic distribution in Hela cells, although it contains two nuclear localization signals (NLSs) in its N-terminus. To search for its substrates and regulatory components, we screened a two-hybrid library by using the full-length hPFTAIRE1 as a bait. Four 14-3-3 isoforms (beta, epsilon, eta, tau) were identified interacting with the hPFTAIRE1. We found a putative 14-3-3 binding consensus motif (RHSSPSS) in the hPFTAIRE1, which overlapped with its second NLS. Deletion of the RHSSPSS motif or substitution of Ser(119) with Ala in the conserved binding motif abolished the specific interaction between the hPFTAIRE1 and the 14-3-3 proteins. The mutant S120A hPFTAIRE1 also showed a weak interaction to the 14-3-3 proteins. The results suggested that the Ser(119) is crucial for the interaction between hPFTAIRE1 and the 14-3-3 proteins. All the hPFTAIRE1 mutants distributed in cytoplasm of Hela cells and human neuroblastoma cells (SH-SY5Y) when fused to the C-terminus of a green fluorescent protein (GFP), indicating that binding with the 14-3-3 proteins does not contribute to the subcellular localization of the hPFTAIRE1, although the binding may be involved in its signaling regulation.
学科主题Cell Biology
类目[WOS]Cell Biology
关键词[WOS]CDK FAMILY-MEMBER ; NERVOUS-SYSTEM ; PHOSPHORYLATION ; LOCALIZATION ; EXPRESSION ; IDENTIFICATION ; SUGGEST ; TESTIS
收录类别SCI
语种英语
WOS记录号WOS:000239296000003
版本出版稿
源URL[http://202.127.25.143/handle/331003/1729]  
专题上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式
GB/T 7714
Gao, YK,Jiang, M,Yang, T,et al. A Cdc2-related protein kinase hPFTAIRE1 from human brain interacting with 14-3-3 proteins[J]. CELL RESEARCH,2006,16(6):539-547.
APA Gao, YK,Jiang, M,Yang, T,Ni, J,&Chen, JY.(2006).A Cdc2-related protein kinase hPFTAIRE1 from human brain interacting with 14-3-3 proteins.CELL RESEARCH,16(6),539-547.
MLA Gao, YK,et al."A Cdc2-related protein kinase hPFTAIRE1 from human brain interacting with 14-3-3 proteins".CELL RESEARCH 16.6(2006):539-547.

入库方式: OAI收割

来源:上海生物化学与细胞生物学研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。