A Cdc2-related protein kinase hPFTAIRE1 from human brain interacting with 14-3-3 proteins
文献类型：期刊论文
| 作者 | Gao, YK; Jiang, M; Yang, T; Ni, J; Chen, JY |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2006 |
| 卷号 | 16期号:6页码:539-547 |
| 关键词 | hPFTAIRE1 14-3-3 proteins 14-3-3 binding motif two-hybrid |
| 通讯作者 | Chen, JY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,jychen@sunm.shcnc.ac.cn |
| 英文摘要 | hPFTAIRE1 (PFTK1), a Cdc2-related protein kinase, is highly expressed in human brain. It exhibits cytoplasmic distribution in Hela cells, although it contains two nuclear localization signals (NLSs) in its N-terminus. To search for its substrates and regulatory components, we screened a two-hybrid library by using the full-length hPFTAIRE1 as a bait. Four 14-3-3 isoforms (beta, epsilon, eta, tau) were identified interacting with the hPFTAIRE1. We found a putative 14-3-3 binding consensus motif (RHSSPSS) in the hPFTAIRE1, which overlapped with its second NLS. Deletion of the RHSSPSS motif or substitution of Ser(119) with Ala in the conserved binding motif abolished the specific interaction between the hPFTAIRE1 and the 14-3-3 proteins. The mutant S120A hPFTAIRE1 also showed a weak interaction to the 14-3-3 proteins. The results suggested that the Ser(119) is crucial for the interaction between hPFTAIRE1 and the 14-3-3 proteins. All the hPFTAIRE1 mutants distributed in cytoplasm of Hela cells and human neuroblastoma cells (SH-SY5Y) when fused to the C-terminus of a green fluorescent protein (GFP), indicating that binding with the 14-3-3 proteins does not contribute to the subcellular localization of the hPFTAIRE1, although the binding may be involved in its signaling regulation. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | CDK FAMILY-MEMBER ; NERVOUS-SYSTEM ; PHOSPHORYLATION ; LOCALIZATION ; EXPRESSION ; IDENTIFICATION ; SUGGEST ; TESTIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000239296000003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1729]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Gao, YK,Jiang, M,Yang, T,et al. A Cdc2-related protein kinase hPFTAIRE1 from human brain interacting with 14-3-3 proteins[J]. CELL RESEARCH,2006,16(6):539-547. |
| APA | Gao, YK,Jiang, M,Yang, T,Ni, J,&Chen, JY.(2006).A Cdc2-related protein kinase hPFTAIRE1 from human brain interacting with 14-3-3 proteins.CELL RESEARCH,16(6),539-547. |
| MLA | Gao, YK,et al."A Cdc2-related protein kinase hPFTAIRE1 from human brain interacting with 14-3-3 proteins".CELL RESEARCH 16.6(2006):539-547. |
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