Tyrosine residues of the extrinsic 23 kDa protein are important for its interaction with spinach PSII membranes
文献类型：期刊论文
| 作者 | Gao, JP; Zhang, F; Weng, J; Ruan, KC; Xu, CH |
| 刊名 | PROTEIN AND PEPTIDE LETTERS
 |
| 出版日期 | 2006 |
| 卷号 | 13期号:6页码:539-544 |
| 关键词 | chemical modification extrinsic 23 kDa protein circular dichroism reconstitution ability |
| 通讯作者 | Xu, CH (reprint author), Chinese Acad Sci, SIBS, Inst Plant Physiol, 345 Lingling Lu, Shanghai 200032, Peoples R China.,xch@iris.sipp.ac.cn |
| 英文摘要 | The 1, 4, and 8 tyrosine (Tyr) residues on the PSII extrinsic 23 kDa protein were modified with 5, 10 or 40 mM N-acetylimidazole (NAI) respectively. The amount of rebound NAI-modified extrinsic 23 kDa protein was 98%, 80%, and 5% of that in the unmodified protein, respectively. These results indicate that the Tyr residues are absolutely essential to reconstitution ability. Further, the fluorescence and circular dichroism spectra among native and NAI-modified extrinsic 23 kDa proteins were similar, suggesting that the modification by NAI did not markedly influence the basic secondary structure of the native conformation. Thus, we have concluded that the tyrosine residues in the extrinsic 23 kDa protein are important for interaction with PSII membranes. In addition, we found that the structure of the extrinsic 23 kDa protein is stable in suspension (pH 4-9 or T-m 25-55 degrees C). |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | OXYGEN-EVOLVING COMPLEX ; MANGANESE-STABILIZING PROTEIN ; PHOTOSYSTEM-II ; 33-KDA PROTEIN ; ELECTROSTATIC INTERACTION ; THERMAL INACTIVATION ; N-ACETYLIMIDAZOLE ; EVOLUTION ; CYANOBACTERIUM ; IDENTIFICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000238215700002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1735]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Gao, JP,Zhang, F,Weng, J,et al. Tyrosine residues of the extrinsic 23 kDa protein are important for its interaction with spinach PSII membranes[J]. PROTEIN AND PEPTIDE LETTERS,2006,13(6):539-544. |
| APA | Gao, JP,Zhang, F,Weng, J,Ruan, KC,&Xu, CH.(2006).Tyrosine residues of the extrinsic 23 kDa protein are important for its interaction with spinach PSII membranes.PROTEIN AND PEPTIDE LETTERS,13(6),539-544. |
| MLA | Gao, JP,et al."Tyrosine residues of the extrinsic 23 kDa protein are important for its interaction with spinach PSII membranes".PROTEIN AND PEPTIDE LETTERS 13.6(2006):539-544. |
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