Structural analysis of fibroin heavy chain signal peptide of silkworm Bombyx mori
文献类型：期刊论文
| 作者 | Wang, SP; Guo, TQ; Guo, XY; Huang, JT; Lu, CD |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2006 |
| 卷号 | 38期号:7页码:507-513 |
| 关键词 | signal peptide fibroin heavy chain silkworm Bombyx mori rAcMNPV |
| 通讯作者 | Lu, CD (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,cdlu@sibs.ac.cn |
| 英文摘要 | To study the minimal length required for the secretion of recombinant proteins and silk proteins in posterior silk gland, the signal peptide (SP) of the fibroin heavy chain (FibH) of silkworm Bombyx mori was systematically shortened from the C-terminal. Its effect on the secretion of protein was observed using enhanced green fluorescent protein (EGFP) as a reporter. Secretion of EGFP fusion proteins was examined under fluorescence microscope. FibH SPs with lengths of 20, 18, 16 and 12 a.a. can direct the secretion of the reporter, yet those with lengths of 11, 10, 9, 8 and I a.a. can not. When the FibH SP was shortened to 12 a.a., the secretion efficiency was decreased slightly and cleavage occurred within EGFP. When 16 a.a. of the FibH SP were used, the secretion of fusion protein was normal and the cleavage site was between the Gly-Ser linker and Met, the starting amino acid of EGFP. These findings are applicable for the expression of foreign proteins in silkworm silk gland. The cleavage site of the SP is discussed and compared with the predictive results of the SignalP 3.0 online prediction program. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | TRANSGENIC SILKWORMS ; CLEAVAGE SITES ; SEQUENCE ; PROTEINS ; PREDICTION ; GLAND ; GENE ; SECRETION ; MEMBRANES ; SYSTEM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000239121600010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1737]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, SP,Guo, TQ,Guo, XY,et al. Structural analysis of fibroin heavy chain signal peptide of silkworm Bombyx mori[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2006,38(7):507-513. |
| APA | Wang, SP,Guo, TQ,Guo, XY,Huang, JT,&Lu, CD.(2006).Structural analysis of fibroin heavy chain signal peptide of silkworm Bombyx mori.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,38(7),507-513. |
| MLA | Wang, SP,et al."Structural analysis of fibroin heavy chain signal peptide of silkworm Bombyx mori".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 38.7(2006):507-513. |
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