Immortalization of normal human cytotrophoblast cells by reconstitution of telomeric reverse transcriptase activity
文献类型：期刊论文
| 作者 | Wang, Y; Qiu, W; Feng, HC; Li, YX; Zhuang, LZ; Wang, Z; Liu, Y; Zhou, JQ; Zhang, DH; Tsao, GSW |
| 刊名 | MOLECULAR HUMAN REPRODUCTION
 |
| 出版日期 | 2006 |
| 卷号 | 12期号:7页码:451-460 |
| 关键词 | G1/S checkpoint human cytotrophoblast immortalization telomerase |
| 通讯作者 | Wang, Y (reprint author), Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, 25 Beisihuanxi Rd, Beijing 100080, Peoples R China.,wangyl@ioz.ac.cn ; gswtsao@hkucc.hku.hk |
| 英文摘要 | Placental trophoblast cells are unique endocrine cells that play vital roles during the processes of embryonic implantation and placentation. However, research into the function of human trophoblast has been largely restrained mainly due to a lack of adequate cell models. A normal placenta-origin cytotrophoblast cell line (NPC) was previously established by our group, but these cells showed replicating senescence after 50 population doublings (PDs). In this study, the human telomerase catalytic subunit gene (htert) was transferred into B6 strain of NPC cells, and strains with reconstituted telomerase activity (B6Tert) were established. It was shown that B6Tert-1 cells produce various biomarkers of normal extravillous cytotrophoblasts during the early weeks of gestation. Meanwhile, the cell invasiveness was inhibited by transforming growth factor beta (TGF beta). However, their ability to form syncytium was relatively low when stimulated with fetal calf serum (FCS). The cells maintained normal cell growth properties and failed to elicit tumours in nude mice. They proliferated continuously with no signs of senescence until the final count at 210 PDs. The growth rate of B6Tert-1 cells was increased when compared with the parental cells, which results, at least partly, from facilitating release of the G1/S checkpoint during the cell-cycle regulation. This is the first report of immortalizing human normal cytotrophoblast (CTB) cells by activation of telomerase activity. The cells will provide an ideal in vitro model for the study of human extravillous trophoblast (EVT) functions and consequently the mechanisms of embryonic implantation and placentation. |
| 学科主题 | Developmental Biology; Obstetrics & Gynecology; Reproductive Biology |
| 类目[WOS] | Developmental Biology ; Obstetrics & Gynecology ; Reproductive Biology |
| 关键词[WOS] | 1ST TRIMESTER ; LIFE-SPAN ; TISSUE INHIBITORS ; EPITHELIAL-CELLS ; HUMAN-PLACENTA ; EXPRESSION ; CANCER ; ESTABLISHMENT ; TROPHOBLAST ; GROWTH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000239905300006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1742]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Y,Qiu, W,Feng, HC,et al. Immortalization of normal human cytotrophoblast cells by reconstitution of telomeric reverse transcriptase activity[J]. MOLECULAR HUMAN REPRODUCTION,2006,12(7):451-460. |
| APA | Wang, Y.,Qiu, W.,Feng, HC.,Li, YX.,Zhuang, LZ.,...&Tsao, GSW.(2006).Immortalization of normal human cytotrophoblast cells by reconstitution of telomeric reverse transcriptase activity.MOLECULAR HUMAN REPRODUCTION,12(7),451-460. |
| MLA | Wang, Y,et al."Immortalization of normal human cytotrophoblast cells by reconstitution of telomeric reverse transcriptase activity".MOLECULAR HUMAN REPRODUCTION 12.7(2006):451-460. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。