Quantitative investigation of the interaction between granulocyte-macrophage colonystimulating factor and heparin by capillary zone ellectrophoresis
文献类型：期刊论文
| 作者 | Liang, AY; Du, YG; Wang, KY; Lin, BC |
| 刊名 | JOURNAL OF SEPARATION SCIENCE
 |
| 出版日期 | 2006 |
| 卷号 | 29期号:11页码:1637-1641 |
| 关键词 | capillary zone electrophoresis granulocyte-macrophage colony-stimulating factor heparin interaction |
| 通讯作者 | Lin, BC (reprint author), Chinese Acad Sci, Dalian Inst Chem Phys, 457 Zhongshan Rd, Dalian 116023, Peoples R China.,bclin@dicp.ac.cn |
| 英文摘要 | The interactions between granulocyte-macrophage colony-stimulating factor (GMCSF) and heparin or low-molecular weight heparin (LMWH) were studied by CZE. It was found that GM-CSF could bind to both heparin and LMWH. The binding constants were calculated from Scatchard regression to be (6.5 +/- 0.8) x 10(5)/M and (11.2 +/- 0.7) x 10(5)/M, respectively. The specificity of the interaction between GM-CSF and heparin was also studied by employing another sulfated K carrageenan oligosaccharide as a control. Results showed that K carrageenan oligosaccharide could not interact with GM-CSF, indicating that GM-CSF could specifically interact with heparin. |
| 学科主题 | Chemistry |
| 类目[WOS] | Chemistry, Analytical |
| 关键词[WOS] | COLONY-STIMULATING FACTOR ; FACTOR GM-CSF ; ELECTROPHORESIS ; BINDING ; GLYCOSAMINOGLYCANS ; AFFINITY ; SULFATE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000239574100014 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1776]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liang, AY,Du, YG,Wang, KY,et al. Quantitative investigation of the interaction between granulocyte-macrophage colonystimulating factor and heparin by capillary zone ellectrophoresis[J]. JOURNAL OF SEPARATION SCIENCE,2006,29(11):1637-1641. |
| APA | Liang, AY,Du, YG,Wang, KY,&Lin, BC.(2006).Quantitative investigation of the interaction between granulocyte-macrophage colonystimulating factor and heparin by capillary zone ellectrophoresis.JOURNAL OF SEPARATION SCIENCE,29(11),1637-1641. |
| MLA | Liang, AY,et al."Quantitative investigation of the interaction between granulocyte-macrophage colonystimulating factor and heparin by capillary zone ellectrophoresis".JOURNAL OF SEPARATION SCIENCE 29.11(2006):1637-1641. |
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