Identification of breast cancer metastasis-associated proteins in an isogenic tumor metastasis model using two-dimensional gel electrophoresis and liquid chromatography-ion trap-mass spectrometry
文献类型：期刊论文
| 作者 | Li, DQ; Wang, L; Fei, F; Hou, YF; Luo, JM; Zeng, WCR; Wu, X; Lu, JS; Di, GH; Ou, ZL |
| 刊名 | PROTEOMICS
 |
| 出版日期 | 2006 |
| 卷号 | 6期号:11页码:3352-3368 |
| 关键词 | breast cancer mass spectrometry metastasis two-dimensional gel electrophoresis |
| 通讯作者 | Shao, ZM (reprint author), Fudan Univ, Canc Hosp, Inst Canc, Dept Breast Surg,Breast Canc Inst, 270 Dong An Rd, Shanghai 200032, Peoples R China.,zhimingshao@yahoo.com |
| 英文摘要 | To better understand the molecular mechanisms underlying breast cancer metastasis and search for potential markers for metastatic progression, we have developed a highly metastatic variant of human MDA-MB-435 breast cancer cell line through in vivo stepwise selection of pulmonary metastatic cells caused by parental MDA-MB-435 cells in the athymic mice. Comparative proteomic analysis using 2-DE and LC-IT-MS revealed that 102 protein spots were reproducibly altered more than three-fold between the selected variant and its parental counterpart. Eleven differentially expressed protein spots were identified with high confidence using SEQUEST with uninterpreted tandem mass raw data. Cathepsin D precursor, peroxiredoxin 6 (PDX6), heat shock protein 27 (HSP27), HSP60, tropomyosin 1 (TPM1), TPM2, TPM3, TPM4,14-3-3 protein epsilon, and tumor protein D54 were up-regulated in the highly metastatic variant, whereas alpha B-crystalline (CRAB) was only detected in its parental counterpart. Differential expression was confirmed for four proteins including PDX6, CRAB, TPM4, and HSP60 by real-time quantitative PCR and Western blotting analysis in our model. Immunohistochemical analysis in 80 breast cancer donors demonstrated a significant association of TPM4 (p = 0.002), HSP60 (p = 0.001), PDX6 (p = 0.002) but not CRAB (p = 0.113) staining with the presence of lymph node metastasis. In addition, TPM4 staining was also associated with clinical stage (p = 0.000), but no significant association was found between TPM4, PDX6, CRAB, and HSP60 expression and tumor size, hormone receptor, and HER-2 status (p > 0.05). The functional implication of these identified proteins was also discussed. These proteomic data are valuable and informative for understanding breast cancer metastasis and searching for potential markers for metastatic progression. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology |
| 关键词[WOS] | ALPHA-B-CRYSTALLIN ; CARCINOMA-CELL-LINES ; GENE-EXPRESSION ; PROTEOMIC ANALYSIS ; ELECTROSPRAY IONIZATION ; CLINICAL-SIGNIFICANCE ; MDA-MB-435 CELLS ; MESSENGER-RNA ; CATHEPSIN-D ; TROPOMYOSIN-1 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000238333400011 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1777]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, DQ,Wang, L,Fei, F,et al. Identification of breast cancer metastasis-associated proteins in an isogenic tumor metastasis model using two-dimensional gel electrophoresis and liquid chromatography-ion trap-mass spectrometry[J]. PROTEOMICS,2006,6(11):3352-3368. |
| APA | Li, DQ.,Wang, L.,Fei, F.,Hou, YF.,Luo, JM.,...&Shao, ZM.(2006).Identification of breast cancer metastasis-associated proteins in an isogenic tumor metastasis model using two-dimensional gel electrophoresis and liquid chromatography-ion trap-mass spectrometry.PROTEOMICS,6(11),3352-3368. |
| MLA | Li, DQ,et al."Identification of breast cancer metastasis-associated proteins in an isogenic tumor metastasis model using two-dimensional gel electrophoresis and liquid chromatography-ion trap-mass spectrometry".PROTEOMICS 6.11(2006):3352-3368. |
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