Interactions of dextran sulfates with granulocyte colony-stimulating factor and their effects on leukemia cells
文献类型：期刊论文
| 作者 | Liang, AY; Zhou, XM; Wang, QY; Liu, X; Qin, JH; Du, YG; Wang, KY; Lin, BC |
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2006 |
| 卷号 | 27期号:16页码:3195-3201 |
| 关键词 | capillary zone electrophoresis dextran sulfate granulocyte colony-stimulating factor interaction leukemia cells |
| 通讯作者 | Lin, BC (reprint author), Chinese Acad Sci, Dalian Inst Chem Phys, 457 Zhongshan Rd, Dalian 116023, Peoples R China.,bclin@dicp.ac.cn |
| 英文摘要 | The interactions between granulocyte colony-stimulating factor (G-CSF) and dextran sulfate (DS) with different chain lengths and sulfate contents were studied by capillary zone electrophoresis. It was found that DS with a molecular mass of 500 kDa (DS500) could bind to G-CSF and the binding constant and binding sites were determined using Scatchard plot to be 1.17 x 10(6) M(-1) and 8, respectively. DS with a molecular mass of 40 kDa also had the affinity to G-CSF and the binding constant and binding sties were 1.01 x 10(6) M-1 and 8, respectively. However, DS with a molecular mass of 8 kDa and the non-sulfated saccharide, dextran, had no affinity to G-CSF The results indicate that the interactions between G-CSIF and DS are dependent on the chain lengths and sulfate contents of the saccharides. In addition, the effects of G-CSF-binding DS on a G-CSF-dependent leukemia cell line were investigated using biological methods. Results show that DS500 plus G-CSF has potential therapeutic effect on cancers because their combination could inhibit the growth and induce the differentiation of the leukemia cells. |
| 学科主题 | Biochemistry & Molecular Biology; Chemistry |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 关键词[WOS] | FIBROBLAST GROWTH-FACTOR ; HEPARAN-SULFATE ; CAPILLARY-ELECTROPHORESIS ; BINDING ; PROTEOGLYCANS ; DIFFERENTIATION ; RECEPTOR ; COMPLEX ; PROTEIN ; NFS-60 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000240333600005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1788]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liang, AY,Zhou, XM,Wang, QY,et al. Interactions of dextran sulfates with granulocyte colony-stimulating factor and their effects on leukemia cells[J]. ELECTROPHORESIS,2006,27(16):3195-3201. |
| APA | Liang, AY.,Zhou, XM.,Wang, QY.,Liu, X.,Qin, JH.,...&Lin, BC.(2006).Interactions of dextran sulfates with granulocyte colony-stimulating factor and their effects on leukemia cells.ELECTROPHORESIS,27(16),3195-3201. |
| MLA | Liang, AY,et al."Interactions of dextran sulfates with granulocyte colony-stimulating factor and their effects on leukemia cells".ELECTROPHORESIS 27.16(2006):3195-3201. |
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