Syntaxin 1A promotes the endocytic sorting of EAAC1 leading to inhibition of glutamate transport
文献类型：期刊论文
| 作者 | Yu, YX; Shen, L; Xia, P; Tang, YW; Bao, L; Pei, G |
| 刊名 | JOURNAL OF CELL SCIENCE
 |
| 出版日期 | 2006 |
| 卷号 | 119期号:18页码:3776-3787 |
| 关键词 | EAAC1 syntaxin 1A endocytic sorting glutamate transport kainic acid surface expression |
| 通讯作者 | Bao, L (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Mol Cell Biol Lab, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,baolan@sibs.ac.cn ; gpei@sibs.ac.cn |
| 英文摘要 | The neuronal glutamate transporter, excitatory amino-acid carrier 1 (EAAC1), plays an important role in the modulation of neurotransmission and contributes to synthesis of the inhibitory neurotransmitter gamma-aminobutyric acid ( GABA) and to epileptogenesis. However, the mechanisms that regulate EAAC1 endocytic sorting and function remain largely unknown. Here, we first demonstrate that EAAC1 undergoes internalization through the clathrin-mediated pathway and further show that syntaxin 1A, a key molecule in synaptic exocytosis, potentiates EAAC1 internalization, thus leading to the functional inhibition of EAAC1. In the presence of the transmembrane domain of syntaxin 1A, its H3 coiled-coil domain of syntaxin 1A is necessary and sufficient for the inhibition of EAAC1. Furthermore, specific suppression of endogenous syntaxin 1A significantly blocked EAAC1 endocytic sorting and lysosomal degradation promoted by kainic acid, a drug for kindling the animal model of human temporal lobe epilepsy in rat, indicating a potential role of syntaxin 1A in epileptogenesis. These findings provide new evidence that syntaxin 1A serves as an intrinsic enhancer to EAAC1 endocytic sorting and further suggest that syntaxin 1A is conversant with both 'ins' and 'outs' of synaptic neurotransmission. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | ACID CARRIER-1 EAAC1 ; PROTEIN-KINASE-C ; NEUROTRANSMITTER TRANSPORTERS ; REGULATED TRAFFICKING ; SYNAPTIC LOCALIZATION ; ALZHEIMERS-DISEASE ; GABA TRANSPORTERS ; RAT HIPPOCAMPUS ; XENOPUS-OOCYTES ; MUSCLE-CELLS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000240386300007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1792]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yu, YX,Shen, L,Xia, P,et al. Syntaxin 1A promotes the endocytic sorting of EAAC1 leading to inhibition of glutamate transport[J]. JOURNAL OF CELL SCIENCE,2006,119(18):3776-3787. |
| APA | Yu, YX,Shen, L,Xia, P,Tang, YW,Bao, L,&Pei, G.(2006).Syntaxin 1A promotes the endocytic sorting of EAAC1 leading to inhibition of glutamate transport.JOURNAL OF CELL SCIENCE,119(18),3776-3787. |
| MLA | Yu, YX,et al."Syntaxin 1A promotes the endocytic sorting of EAAC1 leading to inhibition of glutamate transport".JOURNAL OF CELL SCIENCE 119.18(2006):3776-3787. |
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