Characterization of novel M-superfamily conotoxins with new disulfide linkage
文献类型：期刊论文
| 作者 | Han, YH; Wang, Q; Jiang, H; Liu, L; Xiao, C; Yuan, DD; Shao, XX; Dai, QY; Cheng, JS; Chi, CW |
| 刊名 | FEBS JOURNAL
 |
| 出版日期 | 2006 |
| 卷号 | 273期号:21页码:4972-4982 |
| 关键词 | cDNA cloning disulfide connectivity M-1 branch M-superfamily conotoxin protein purification |
| 通讯作者 | Chi, CW (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Biochem & Cell Biol, Inst Biochem & Cell Biol,Key Lab Proteom, 320 YueYang Rd, Shanghai 200031, Peoples R China.,chi@sunm.shcnc.ac.cn |
| 英文摘要 | The M-superfamily with the typical Cys framework (-CC-C-C-CC-) is one of the seven major superfamilies of conotoxins found in the venom of cone snails. Based on the number of residues in the last Cys loop (between C4 and C5), M-superfamily conotoxins can be provisionally categorized into four branches (M-1, M-2, M-3, M-4) [Corpuz GP, Jacobsen RB, Jimenez EC, Watkins M, Walker C, Colledge C, Garrett JE, McDougal O, Li W, Gray WR, et al. (2005) Biochemistry44, 8176-8186]. Here we report the purification of seven M-superfamily conotoxins from Conus marmoreus (five are novel and two are known as mr3a and mr3b) and one known M-1 toxin tx3a from Conus textile. In addition, six novel cDNA sequences of M-superfamily conotoxins have been identified from C. marmoreus, Conus leopardus and Conus quercinus. Most of the above novel conotoxins belong to M-1 and M-2 and only one to M-3. The disulfide analyses of two M-1 conotoxins, mr3e and tx3a, revealed that they possess a new disulfide bond arrangement (C1-C5, C2-C4, C3-C6) which is different from those of the M-4 branch (C1-C4, C2-C5, C3-C6) and M-2 branch (C1-C6, C2-C4, C3-C5). This newly characterized disulfide connectivity was confirmed by comparing the HPLC profiles of native mr3e and its two regioselectively folded isoforms. This is the first report of three different patterns of disulfide connectivity in conotoxins with the same cysteine framework. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | RESISTANT SODIUM-CHANNELS ; CONUS VENOMS ; K+ CHANNEL ; PEPTIDES ; INHIBITOR ; DIVERSITY ; SEQUENCE ; RECEPTOR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000241343900014 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1798]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Han, YH,Wang, Q,Jiang, H,et al. Characterization of novel M-superfamily conotoxins with new disulfide linkage[J]. FEBS JOURNAL,2006,273(21):4972-4982. |
| APA | Han, YH.,Wang, Q.,Jiang, H.,Liu, L.,Xiao, C.,...&Chi, CW.(2006).Characterization of novel M-superfamily conotoxins with new disulfide linkage.FEBS JOURNAL,273(21),4972-4982. |
| MLA | Han, YH,et al."Characterization of novel M-superfamily conotoxins with new disulfide linkage".FEBS JOURNAL 273.21(2006):4972-4982. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。