beta-catenin relieves I-mfa-mediated suppression of LEF1 in mammalian cells
文献类型：期刊论文
| 作者 | Pan, WJ; Jia, YY; Huang, T; Wang, JY; Tao, DL; Gan, XQ; Li, L |
| 刊名 | JOURNAL OF CELL SCIENCE
 |
| 出版日期 | 2006 |
| 卷号 | 119期号:23页码:4850-4856 |
| 关键词 | Wnt beta-catenin I-mfa LEF-1 myogenesis p19 carcinoma stem cells |
| 通讯作者 | Li, L (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,lli@sibs.ac.cn |
| 英文摘要 | We have previously shown that beta-catenin interacts with a transcription suppressor I-mfa and, through this interaction, canonical Wnt signaling could relieve I-mfa-mediated suppression of myogenic regulatory factors (MRFs). In this study, we found that, based on this interaction, I-mfa-mediated suppression of the Wnt transcription factor T-cell factor/lymphoid enhancing factor-1 (TCF/LEF-1) can also be relieved. Our work showed that knocking down endogenous I-mfa expression mimics canonical Wnt treatment by inducing myogenesis and increasing Wnt reporter gene activity, endogenous Wnt target gene expression and expression of MRFs in P19 cells. More importantly, these I-mfa small interfering RNA (siRNA)-induced effects could be blocked by a dominant-negative mutant of LEF-1, confirming the involvement of the TCF/LEF-1 pathway. In addition, we found that beta-catenin could compete with I-mfa for binding to LEF-1 and relieve the inhibitory effects of I-mfa in overexpression systems. Furthermore, canonical Wnt was able to reduce the levels of endogenous I-mfa associated with LEF-1, while increasing that of I-mfa associated with beta-catenin. All of the evidence supports a conclusion that I-mfa can suppress myogenesis by inhibiting TCF/LEF-1 and that canonical Wnt signaling may relieve the suppression through elevating beta-catenin levels, which in turn relieve I-mfa-mediated suppression. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | MYOGENIC REGULATORY FACTORS ; WNT SIGNALING PATHWAY ; TRANSCRIPTION FACTORS ; SKELETAL MYOGENESIS ; CARCINOMA-CELLS ; DOMAIN PROTEINS ; MYOD FAMILY ; P19 CELLS ; CYCLIN D1 ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000242995800008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1801]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Pan, WJ,Jia, YY,Huang, T,et al. beta-catenin relieves I-mfa-mediated suppression of LEF1 in mammalian cells[J]. JOURNAL OF CELL SCIENCE,2006,119(23):4850-4856. |
| APA | Pan, WJ.,Jia, YY.,Huang, T.,Wang, JY.,Tao, DL.,...&Li, L.(2006).beta-catenin relieves I-mfa-mediated suppression of LEF1 in mammalian cells.JOURNAL OF CELL SCIENCE,119(23),4850-4856. |
| MLA | Pan, WJ,et al."beta-catenin relieves I-mfa-mediated suppression of LEF1 in mammalian cells".JOURNAL OF CELL SCIENCE 119.23(2006):4850-4856. |
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