Coimmunization with IL-15 plasmid enhances the longevity of CD8 T cells induced by DNA encoding hepatitis B virus core antigen
文献类型：期刊论文
| 作者 | Zhang, W; Dong, SF; Sun, SH; Wang, Y; Li, GD; Di, Q |
| 刊名 | WORLD JOURNAL OF GASTROENTEROLOGY
 |
| 出版日期 | 2006 |
| 卷号 | 12期号:29页码:4727-4735 |
| 关键词 | vaccine DNA vaccine hepatitis B virus core antigen |
| 通讯作者 | Di, Q (reprint author), Fudan Univ, Shanghai Med Coll, Key Lab Med Mol Virol, Shanghai 200032, Peoples R China.,dqu@shmu.edu.cn |
| 英文摘要 | AIM: To test the feasibility of delivering a plasmid encoding IL-15 as a DNA vaccine adjuvant for improving the immune responses induced by hepatitis B virus core gene DNA vaccine. METHODS: We used RT-PCR based strategies to develop IL-15 expression constructs. We first confirmed that the gene could be expressed in Escherichia coli due to the poor expression of IL-15. Then the bioactivity of IL-15 plasmid expression product was identified by CTLL-2 proliferation assay. One hundred micrograms of DNA from each of the IL-15 eukaryotic expressed plasmid and the recombinant plasmid harboring DNA encoding the 144 amino acids of the N-terminus of HBV core gene (abbreviated pHBc144) was used to co-immunize C57 BL/6 mice. The titer of anti-HBcIgG was detected by ELISA and the antigen-specific CD8(+)T cells (CD8(+)IFN-gamma(+) T cells) were detected by intracellular cytokine staining at different time points. RESULTS: After co-immunization by pIL-15 and pHBc144 DNA vaccine the antigen-specific CD8(+) cells of mice increased gradually, the first peak of immune response appeared 14 d later, then the number of antigen-specific CD8(+) Ts cells decreased gradually and maintained at a steady level in 3 mo. After boosting, the number of antigen-specific CD8(+)T cells reached the second peak 10 d later with a double of the 1st peak, then the number of antigen-specific CD8(+)T cells decreased slowly. IL-15 as a gene adjuvant had no significant effect on humoral immune responses induced by hepatitis B virus core gene DNA vaccine, but increased the memory antigen-specific CD8(+) T cells induced by hepatitis B virus core gene DNA vaccine. CONCLUSION: DNA vaccine constructed by HBc Ag 1-144 amino acid induces effective cell immunity, and cytokine plasmid-delivered IL-15 enhances the longevity of CD8(+)T cells. (c) 2006 The WJG Press. All rights reserved. |
| 学科主题 | Gastroenterology & Hepatology |
| 类目[WOS] | Gastroenterology & Hepatology |
| 关键词[WOS] | SIGNAL PEPTIDES ; VIRAL CONTROL ; INFECTION ; ISOFORMS ; SECRETION ; MICE ; INTERLEUKIN-15 ; RESPONSES ; ADJUVANT ; SEQUENCE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000239949900020 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1803]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, W,Dong, SF,Sun, SH,et al. Coimmunization with IL-15 plasmid enhances the longevity of CD8 T cells induced by DNA encoding hepatitis B virus core antigen[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2006,12(29):4727-4735. |
| APA | Zhang, W,Dong, SF,Sun, SH,Wang, Y,Li, GD,&Di, Q.(2006).Coimmunization with IL-15 plasmid enhances the longevity of CD8 T cells induced by DNA encoding hepatitis B virus core antigen.WORLD JOURNAL OF GASTROENTEROLOGY,12(29),4727-4735. |
| MLA | Zhang, W,et al."Coimmunization with IL-15 plasmid enhances the longevity of CD8 T cells induced by DNA encoding hepatitis B virus core antigen".WORLD JOURNAL OF GASTROENTEROLOGY 12.29(2006):4727-4735. |
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