Transactivated minimal E1b promoter is capable of driving the expression of short hairpin RNA
文献类型：期刊论文
| 作者 | Huang, M; Jia, FJ; Yan, YC; Guo, LH; Li, YP |
| 刊名 | JOURNAL OF VIROLOGICAL METHODS
 |
| 出版日期 | 2006 |
| 卷号 | 134期号:1页码:48-54 |
| 关键词 | RNA interference shRNA RNA polymerase II E1b promoter Gal4/UAS system |
| 通讯作者 | Guo, LH (reprint author), Chinese Acad Sci, Grad Sch, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol,Lab Mol Cell Biol, Shanghai, Peoples R China.,guolihe@celstar.com.cn ; oocyte@sunm.shcnc.ac.cn |
| 英文摘要 | The strategy that transcribes short hairpin RNAs (shRNAs) by RNA polymerase 11 promoters is expected to present flexible approaches for regulating the patterns of shRNA expression. The capacity of generating shRNA by a modified adenovirus RNA polymerase II E1b promoter was studied. This 49 bp promoter consists of a TATA-box and an initiation element. It is demonstrated that this modified E1b promoter is capable of driving shRNA transcription and causing either long-term suppression against the target gene in response to the transactivation of constitutively expressed Gal4-VP16 fusion protein or inducible suppression given that the expression of Gal4-VP16 is subject to a dexamethasone inducer. (c) 2005 Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Virology |
| 类目[WOS] | Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology |
| 关键词[WOS] | MAMMALIAN-CELLS ; IN-VIVO ; GENE ; INTERFERENCE ; SIRNA ; INHIBITION ; ZEBRAFISH ; HIV-1 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000238005500007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1813]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Huang, M,Jia, FJ,Yan, YC,et al. Transactivated minimal E1b promoter is capable of driving the expression of short hairpin RNA[J]. JOURNAL OF VIROLOGICAL METHODS,2006,134(1):48-54. |
| APA | Huang, M,Jia, FJ,Yan, YC,Guo, LH,&Li, YP.(2006).Transactivated minimal E1b promoter is capable of driving the expression of short hairpin RNA.JOURNAL OF VIROLOGICAL METHODS,134(1),48-54. |
| MLA | Huang, M,et al."Transactivated minimal E1b promoter is capable of driving the expression of short hairpin RNA".JOURNAL OF VIROLOGICAL METHODS 134.1(2006):48-54. |
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