Mitochondria and calpains mediate caspase-dependent apoptosis induced by doxycycline in HeLa cells
文献类型：期刊论文
| 作者 | Wu, J; Liu, T; Xie, J; Xin, F; Guo, L |
| 刊名 | CELLULAR AND MOLECULAR LIFE SCIENCES
 |
| 出版日期 | 2006 |
| 卷号 | 63期号:1页码:949-957 |
| 关键词 | doxycycline apoptosis mitochondria calpain caspase |
| 通讯作者 | Guo, L (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Room 901,Cell Biol Bldg,320 Yueyang Rd, Shanghai 200031, Peoples R China.,lhguo@sibs.ac.cn |
| 英文摘要 | Doxycycline (Dc) has been demonstrated to inhibit cell growth and induce apoptosis in tumor cells, although its mechanism of action is not fully understood. The present study demonstrates that apoptosis can be induced in HeLa cells. Western blot data demonstrated that cytochrome c (Cyt c), Smac (the second mitochondria-derived activator of caspase), calpain I, caspase-9, -3 and -8 were involved in the apoptotic process, while the pan caspase inhibitor zVAD-fmk almost completely inhibited Dc-induced apoptosis. We further demonstrated that the release of mitochondrial proteins and the activation of calpains occurred upstream of the caspase cascade, in which caspase-9 was activated in response to the release of Cyt c, that caspase-8 activation was caspase and calpain dependent, and that caspase-3 was activated mainly by caspase-8 and -9. Caspase-8 played important roles in the activation of caspase-3 and induction of apoptosis, whereas the role of the caspase-9 was limited. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | CYTOCHROME-C ; SERINE-PROTEASE ; CANCER CELLS ; DEATH DOMAIN ; IN-VITRO ; ACTIVATION ; INHIBITION ; INTERACTS ; CLEAVAGE ; SMAC |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000237359800018 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1817]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wu, J,Liu, T,Xie, J,et al. Mitochondria and calpains mediate caspase-dependent apoptosis induced by doxycycline in HeLa cells[J]. CELLULAR AND MOLECULAR LIFE SCIENCES,2006,63(1):949-957. |
| APA | Wu, J,Liu, T,Xie, J,Xin, F,&Guo, L.(2006).Mitochondria and calpains mediate caspase-dependent apoptosis induced by doxycycline in HeLa cells.CELLULAR AND MOLECULAR LIFE SCIENCES,63(1),949-957. |
| MLA | Wu, J,et al."Mitochondria and calpains mediate caspase-dependent apoptosis induced by doxycycline in HeLa cells".CELLULAR AND MOLECULAR LIFE SCIENCES 63.1(2006):949-957. |
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