Different fates of donor mitochondrial DNA in bovine-rabbit and cloned bovine-rabbit reconstructed embryos during preimplantation development
文献类型：期刊论文
| 作者 | Jiang, Y; Chen, T; Wang, K; Jiang, MX; Liu, SZ; Ouyang, YC; Sun, QY; Chen, DY |
| 刊名 | FRONTIERS IN BIOSCIENCE
 |
| 出版日期 | 2006 |
| 卷号 | 11期号:1页码:1425-1432 |
| 关键词 | nuclear transfer mtDNA embryo development real time PCR |
| 通讯作者 | Chen, DY (reprint author), Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beisihuanxi Rd 25, Beijing 100080, Peoples R China.,chendy@ioz.ac.cn |
| 英文摘要 | The functions of mitochondria depend on precise interaction between nuclear and cytoplasmic genomes. Non-balance of mtDNA has been reported in most nuclear transfer embryos and offspring. The reason of the degradation of donor mtDNA is still not clear. To further investigate the mechanism, in this study, we designed an experiment as follows. Two fibroblast cell lines sharing same nuclear genome but different mitochondria genome backgrounds, namely cells from ear tissues of cloned bovine and its donor, were choose as donor cells and introduced into enucleated rabbit oocytes. Similar developmental potential was observed in cloned bovine-rabbit (clone group) and bovine-rabbit (non-clone group) embryos. Real-time PCR assay showed that, in non-clone group, bovine mtDNA decreased during the development of reconstructed embryo, and that a sharp decrease was detected at the blastocyst stage. In clone group, bovine mtDNA decreased slightly, and the abrupt reduction of donor mtDNA did not occur during preimplantation development. In addition, an obvious increase in rabbit mtDNA was observed in both groups at the blastocyst stage. Our results demonstrate that: 1) the fates of donor mtDNAs in bovine- rabbit and cloned bovine- rabbit reconstructed embryos were different; and 2) recipient mtDNAs replicate at blastocyst regardless of the difference of donor cells. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | NUCLEAR TRANSFER ; CUMULUS CELLS ; SOMATIC-CELL ; OOCYTES ; HETEROPLASMY ; EMBRYOGENESIS ; FIBROBLASTS ; COMPETENCE ; BIOGENESIS ; CATTLE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000234180200021 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1824]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Jiang, Y,Chen, T,Wang, K,et al. Different fates of donor mitochondrial DNA in bovine-rabbit and cloned bovine-rabbit reconstructed embryos during preimplantation development[J]. FRONTIERS IN BIOSCIENCE,2006,11(1):1425-1432. |
| APA | Jiang, Y.,Chen, T.,Wang, K.,Jiang, MX.,Liu, SZ.,...&Chen, DY.(2006).Different fates of donor mitochondrial DNA in bovine-rabbit and cloned bovine-rabbit reconstructed embryos during preimplantation development.FRONTIERS IN BIOSCIENCE,11(1),1425-1432. |
| MLA | Jiang, Y,et al."Different fates of donor mitochondrial DNA in bovine-rabbit and cloned bovine-rabbit reconstructed embryos during preimplantation development".FRONTIERS IN BIOSCIENCE 11.1(2006):1425-1432. |
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