Dynamic changes in NuMA and microtubules in monkey-rabbit nuclear transfer embryos
文献类型：期刊论文
| 作者 | Yan, LY; Shi, LH; Sheng, HZ; Liu, SZ; Huang, JC; Zhu, ZY; Ouyang, YC; Lei, ZL; Song, XF; Sun, QY |
| 刊名 | FRONTIERS IN BIOSCIENCE
 |
| 出版日期 | 2006 |
| 卷号 | 11期号:1页码:1892-1900 |
| 关键词 | non-human primate somatic cell nuclear transfer first cell cycle nuclear mitotic apparatus protein microtubule activation |
| 通讯作者 | Chen, DY (reprint author), Chinese Acad Sci, State Key Lab Reprod Biol, Inst Zool, 25 Beisihuanxi Rd, Beijing 100080, Peoples R China.,chendy@ioz.ac.cn |
| 英文摘要 | Previous reports have indicated that failure in cloning monkey is attributed to the removal of nuclear mitotic apparatus (NuMA) during enucleation and subsequent abnormal organization of mitotic apparatus. This study investigated the transformation and assembly of tubulin and NuMA protein during the first cell cycle of cloned monkey embryos reconstructed by using enucleated rabbit oocytes as recipients. After the oocyte fused with a fibroblast, extensive microtubule organization was observed around the introduced nucleus in most reconstructed embryos, suggesting the introduction of a somatic cell centrosome. A high proportion of fibroblast nuclei transferred into non-activated oocytes underwent premature chromosome condensation (PCC), transient spindle organization and chromosomes separation, followed by the formation of two pronucleus-like structures. In contrast, fibroblast nuclei in pre-activated ooplasm rarely underwent PCC, but formed a swollen pronucleus-like structure. Normal spindles were observed in about one third of the cloned embryos reconstructed by both methods. After transferring monkey fibroblasts into NuMA-removed enucleated rabbit oocytes, NuMA was localized in pseudo-pronuclei and gradually moved to mitotic spindle poles at the first mitotic spindle poles. NuMA antibody microinjection resulted in spindle disorganization and chromosome misalignment, but did not significantly affect early cleavage. Our findings indicate that: 1. NuMA in donor monkey fibroblast may contribute to form a normal spindle in enucleated rabbit oocyte; 2. when non-activated cytoplasts and pre-activated cytoplasts are used as recipients, the donor nuclei undergo different morphological changes, but yield similar early embryo development; 3. although abnormal spindle organization and chromosome alignment may cause low efficiency of animal cloning, these abnormalities do not significantly affect early cleavage. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | MITOTIC APPARATUS PROTEIN ; OOCYTE ACTIVATION PRIOR ; CUMULUS CELL-NUCLEI ; CLONED EMBRYOS ; CLONING ; FIBROBLASTS ; CENTROSOME ; BLASTOCYST ; EXPRESSION ; PRIMATE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000234180200060 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1825]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yan, LY,Shi, LH,Sheng, HZ,et al. Dynamic changes in NuMA and microtubules in monkey-rabbit nuclear transfer embryos[J]. FRONTIERS IN BIOSCIENCE,2006,11(1):1892-1900. |
| APA | Yan, LY.,Shi, LH.,Sheng, HZ.,Liu, SZ.,Huang, JC.,...&Chen, DY.(2006).Dynamic changes in NuMA and microtubules in monkey-rabbit nuclear transfer embryos.FRONTIERS IN BIOSCIENCE,11(1),1892-1900. |
| MLA | Yan, LY,et al."Dynamic changes in NuMA and microtubules in monkey-rabbit nuclear transfer embryos".FRONTIERS IN BIOSCIENCE 11.1(2006):1892-1900. |
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