Functional disruption of the prion protein gene in cloned goats
文献类型：期刊论文
| 作者 | Yu, GH; Chen, JQ; Yu, HQ; Liu, SG; Chen, J; Xu, XJ; Sha, HY; Zhang, XF; Wu, GX; Xu, SF |
| 刊名 | JOURNAL OF GENERAL VIROLOGY
 |
| 出版日期 | 2006 |
| 卷号 | 87期号:1页码:1019-1027 |
| 通讯作者 | Cheng, GX (reprint author), Shanghai Transgen Res Ctr, 88 Cai Lun Rd, Shanghai 201203, Peoples R China.,chenggx@cngenon.com |
| 英文摘要 | The cellular prion protein (PrPC), a membrane glycoprotein anchored to the outer surface of neurons, lymphocytes and other cells, is associated directly with the pathogenesis of the transmissible spongiform encephalopathies (TSEs) occurring mainly in humans, cattle, sheep and goats. Although mice lacking PrPC develop and reproduce normally and are resistant to scrapie infection, large animals lacking PrPC, especially those species in which TSE occurs naturally, are currently not available. Here, five live PRNP+/- goats cloned by gene targeting are reported. Detailed RNA-transcription and protein-expression analysis of one PRNP+/- goat showed that one allele of the caprine PRNP gene had been disrupted functionally. No gross abnormal development or behaviour could be seen in these PRNP+/- goats up to at least 3 months of age. These heterozygous PRNP+/- goats are ready to be used in producing homozygous PRNP-/- goats in which no PrPC should be expressed. |
| 学科主题 | Biotechnology & Applied Microbiology; Virology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Virology |
| 关键词[WOS] | PRP GENE ; NUCLEAR TRANSFER ; MICE ; SCRAPIE ; CELLS ; SHEEP ; DOPPEL ; ATAXIA ; BRAIN ; PIGS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000236446400033 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1831]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yu, GH,Chen, JQ,Yu, HQ,et al. Functional disruption of the prion protein gene in cloned goats[J]. JOURNAL OF GENERAL VIROLOGY,2006,87(1):1019-1027. |
| APA | Yu, GH.,Chen, JQ.,Yu, HQ.,Liu, SG.,Chen, J.,...&Cheng, GX.(2006).Functional disruption of the prion protein gene in cloned goats.JOURNAL OF GENERAL VIROLOGY,87(1),1019-1027. |
| MLA | Yu, GH,et al."Functional disruption of the prion protein gene in cloned goats".JOURNAL OF GENERAL VIROLOGY 87.1(2006):1019-1027. |
入库方式： OAI收割
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