Dual promoters control the cell-specific expression of the human cell death-inducing DFF45-like effector B gene
文献类型：期刊论文
| 作者 | Da, L; Li, D; Yokoyama, KK; Tsaiping, L; Zhao, MJ |
| 刊名 | BIOCHEMICAL JOURNAL
 |
| 出版日期 | 2006 |
| 卷号 | 393期号:1页码:779-788 |
| 关键词 | cell death-inducing DFF45 (DNA fragmentation factor 45)-like effector (CIDE) cell-specific expression DNA methylation promoter hepatocyte nuclear factor-4 (HNF4) Sp1 |
| 通讯作者 | Zhao, MJ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,mjzhao@sibs.ac.cn |
| 英文摘要 | CIDE-B [cell death-inducing DFF45 (DNA fragmentation factor 45)-like effector B] is a member of the CIDE family of apoptosisinducing factors. The highly restricted pattern of expression of CIDE-B in the liver and spleen suggests that a mechanism exists for the tissue- and cell-specific regulation of transcription of this gene. We have analysed the promoters of the human CIDE-B gene, particularly the mechanism of cell-specific transcription. Expression of CIDE-B is driven by two promoters which are responsible for the synthesis of two types of transcript, and Sp1 and Sp3 are key regulators of basal transcription from both the upstream and the internal promoter, as indicated by EMSAs (electrophoretic mobility-shift assays) and site-directed mutagenesis. Bisulphite sequencing analysis demonstrated that the upstream promoter was hypermethylated in cells that did not express the long transcript of CIDE-B, but was hypomethylated in cells that expressed this transcript. Furthermore, methylation of this region in vitro reduced the promoter activity to similar to 5% of the control. Thus methylation at CpG sites in the upstream promoter region appeared to be important for cell-specific synthesis of the long transcript. By contrast, HNF4 alpha (hepatocyte nuclear factor4 alpha) bound to the internal promoter and enhanced its activity. Moreover, the short transcript of CIDE-B gene was expressed in cells which do not normally express this transcript upon introduction of exogenous HNF4 alpha, demonstrating the involvement of HNF4 alpha in the cell-specific synthesis of the short transcript. Thus our analysis revealed a novel mechanism for the cellspecific transcription of the human CIDE-B gene, which involves epigenetic and genetic control at separate respective promoters. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | DNA-FRAGMENTATION-FACTOR ; ADIPOCYTE-SPECIFIC GENE ; WILD-TYPE P53 ; LEUKOTRIENE B-4 ; CIDE-B ; TRANSCRIPTIONAL REGULATION ; BINDING-PROTEIN ; APOPTOSIS ; METHYLATION ; RECEPTOR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000234947800019 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1834]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Da, L,Li, D,Yokoyama, KK,et al. Dual promoters control the cell-specific expression of the human cell death-inducing DFF45-like effector B gene[J]. BIOCHEMICAL JOURNAL,2006,393(1):779-788. |
| APA | Da, L,Li, D,Yokoyama, KK,Tsaiping, L,&Zhao, MJ.(2006).Dual promoters control the cell-specific expression of the human cell death-inducing DFF45-like effector B gene.BIOCHEMICAL JOURNAL,393(1),779-788. |
| MLA | Da, L,et al."Dual promoters control the cell-specific expression of the human cell death-inducing DFF45-like effector B gene".BIOCHEMICAL JOURNAL 393.1(2006):779-788. |
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