Characterization and application of monoclonal antibodies against N protein of SARS-coronavirus
文献类型：期刊论文
| 作者 | Shang, B; Wang, XY; Yuan, JW; Vabret, A; Wu, XD; Yang, RF; Tian, L; Ji, YY; Deubel, V; Sun, B |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2005 |
| 卷号 | 336期号:1页码:110-117 |
| 关键词 | severe acute respiratory syndrome nucleocapsid protein truncated N protein fragments monoclonal antibody B cell epitopernapping human coronaviruses diagnosis |
| 通讯作者 | Sun, B (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Lab Mol Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,bsun@sibs.ac.cn |
| 英文摘要 | Severe acute respiratory syndrome-coronavirus (SARS-CoV) causes an infectious disease through respiratory route. Diagnosing the disease effectively and accurately at early stage is essential for preventing the disease transmission and performing antiviral treatment. In this study, we raised monoclonal antibodies (mAbs) against the nucleocapsid (N) protein of SARS-CoV and mapped epitopes by using different truncated N protein fragments. The mapping of those epitopes was valuable for constructing pair-Abs used in serological diagnosis. The results showed that all of the six raised mAbs were divided into two groups recognizing the region of amino acids 249-317 (A group) or 317-395 (B group). This region spanning amino acids 249-395 contains predominant B cell epitopes located at the C-terminus of N protein. One pair-Abs, consisting of N protein-specific rabbit polyclonal antibody and SARS-CoV N protein-specific mAb, was selected to construct a sandwich ELISA-kit. The kit was able to specifically detect SARS-CoV N proteins in serum samples. (c) 2005 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | ACUTE RESPIRATORY SYNDROME ; NUCLEOCAPSID PROTEIN ; STRUCTURAL PROTEINS ; ENZYME-IMMUNOASSAY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000231941500018 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1837]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Shang, B,Wang, XY,Yuan, JW,et al. Characterization and application of monoclonal antibodies against N protein of SARS-coronavirus[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2005,336(1):110-117. |
| APA | Shang, B.,Wang, XY.,Yuan, JW.,Vabret, A.,Wu, XD.,...&Sun, B.(2005).Characterization and application of monoclonal antibodies against N protein of SARS-coronavirus.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,336(1),110-117. |
| MLA | Shang, B,et al."Characterization and application of monoclonal antibodies against N protein of SARS-coronavirus".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 336.1(2005):110-117. |
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