Ribosomal protein L10a, a bridge between trichosanthin and the ribosome
文献类型:期刊论文
作者 | Xia, XC; Hou, FJ; Li, J; Nie, HL |
刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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出版日期 | 2005 |
卷号 | 336期号:1页码:281-286 |
关键词 | trichosanthin L10a interact mass spectrometry binding assays surface plasmon resonance |
通讯作者 | Nie, HL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,hlnie@sibs.ac.cn |
英文摘要 | Trichosanthin is a type I ribosome-inactivating protein (RIP) with many pharmacological activities. The trichosanthin-coupled Sepharose affinity purification revealed a protein, which was identified by mass spectrometry as the ribosomal protein L10a. The interaction between trichosanthin and recombinant L10a was further confirmed by in vitro binding assay. Kinetic analysis by surface plasmon resonance technology revealed that L10a had a high affinity to trichosanthin with a K-D of 7.78 nM. The study with mutated forms of trichosanthin demonstrated that this specific association correlates with the ribosome-inactivating activity of trichosanthin. This finding might provide insight into the mechanisms by which trichosanthin inactivates ribosome and that underlies its pharmacological effect. (c) 2005 Published by Elsevier Inc. |
学科主题 | Biochemistry & Molecular Biology; Biophysics |
类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
关键词[WOS] | RICIN-A-CHAIN ; HIV DISEASE ; CELLS ; MECHANISM ; CSA-19 ; CDNA |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000231941500041 |
版本 | 出版稿 |
源URL | [http://202.127.25.143/handle/331003/1838] ![]() |
专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
推荐引用方式 GB/T 7714 | Xia, XC,Hou, FJ,Li, J,et al. Ribosomal protein L10a, a bridge between trichosanthin and the ribosome[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2005,336(1):281-286. |
APA | Xia, XC,Hou, FJ,Li, J,&Nie, HL.(2005).Ribosomal protein L10a, a bridge between trichosanthin and the ribosome.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,336(1),281-286. |
MLA | Xia, XC,et al."Ribosomal protein L10a, a bridge between trichosanthin and the ribosome".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 336.1(2005):281-286. |
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