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Ribosomal protein L10a, a bridge between trichosanthin and the ribosome

文献类型：期刊论文

作者Xia, XC; Hou, FJ; Li, J; Nie, HL
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
出版日期2005
卷号336期号:1页码:281-286
关键词trichosanthin L10a interact mass spectrometry binding assays surface plasmon resonance
通讯作者Nie, HL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,hlnie@sibs.ac.cn
英文摘要Trichosanthin is a type I ribosome-inactivating protein (RIP) with many pharmacological activities. The trichosanthin-coupled Sepharose affinity purification revealed a protein, which was identified by mass spectrometry as the ribosomal protein L10a. The interaction between trichosanthin and recombinant L10a was further confirmed by in vitro binding assay. Kinetic analysis by surface plasmon resonance technology revealed that L10a had a high affinity to trichosanthin with a K-D of 7.78 nM. The study with mutated forms of trichosanthin demonstrated that this specific association correlates with the ribosome-inactivating activity of trichosanthin. This finding might provide insight into the mechanisms by which trichosanthin inactivates ribosome and that underlies its pharmacological effect. (c) 2005 Published by Elsevier Inc.
学科主题Biochemistry & Molecular Biology; Biophysics
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]RICIN-A-CHAIN ; HIV DISEASE ; CELLS ; MECHANISM ; CSA-19 ; CDNA
收录类别SCI
语种英语
WOS记录号WOS:000231941500041
版本出版稿
源URL[http://202.127.25.143/handle/331003/1838]  
专题上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式
GB/T 7714		 Xia, XC,Hou, FJ,Li, J,et al. Ribosomal protein L10a, a bridge between trichosanthin and the ribosome[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2005,336(1):281-286.
APA Xia, XC,Hou, FJ,Li, J,&Nie, HL.(2005).Ribosomal protein L10a, a bridge between trichosanthin and the ribosome.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,336(1),281-286.
MLA Xia, XC,et al."Ribosomal protein L10a, a bridge between trichosanthin and the ribosome".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 336.1(2005):281-286.

入库方式： OAI收割

来源：上海生物化学与细胞生物学研究所

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