Effects of segment substitution on the structure and stability of immunoglobulin G binding domain of streptococcal protein G
文献类型：期刊论文
| 作者 | Du, HN; Zhang, TY; Chang, YG; Lin, DH; Hu, HY |
| 刊名 | BIOPOLYMERS
 |
| 出版日期 | 2005 |
| 卷号 | 79期号:1页码:42264 |
| 关键词 | immunoglobulin G binding domain segment substitution structure stability tertiary interaction |
| 通讯作者 | Hu, HY (reprint author), Chinese Acad Sci, Key Lab Proteom, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,hyhu@sibs.ac.cn |
| 英文摘要 | Structural formation of segments plays pivotal roles in protein folding and stability, but how the segment influences the structural ensemble remains elusive. We engineered two hybrid-proteins by replacing the central helical segment of immunoglobulin G binding domain of streptococcal protein G with an alpha-helix or beta(2)-strand element of a structural homologue, the immunoglobulin G binding domain of streptococcal protein L. The results show that substitution by the a-helical sequence retains a folded structure predominantly with a three-stranded beta-sheet but slightly destabilizes the compact ensemble, while substitution by the beta(2)-strand sequence completely destroys the structural formation. The finding implies that the local segment may influence the tertiary structure and overall stability, and the tertiary interactions may modulate structural formation of the segment, which might be considered when studying protein folding, prediction, design, and engineering. (c) 2005 Wiley Periodicals, Inc. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | DIFFERENT CONFORMATIONS ; BETA-HAIRPIN ; FLUORESCENCE ; TRYPTOPHAN ; SEQUENCE ; SURFACE ; DYNAMICS ; FRAGMENT ; PEPTIDE ; HELIX |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000231427500002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1839]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Du, HN,Zhang, TY,Chang, YG,et al. Effects of segment substitution on the structure and stability of immunoglobulin G binding domain of streptococcal protein G[J]. BIOPOLYMERS,2005,79(1):42264. |
| APA | Du, HN,Zhang, TY,Chang, YG,Lin, DH,&Hu, HY.(2005).Effects of segment substitution on the structure and stability of immunoglobulin G binding domain of streptococcal protein G.BIOPOLYMERS,79(1),42264. |
| MLA | Du, HN,et al."Effects of segment substitution on the structure and stability of immunoglobulin G binding domain of streptococcal protein G".BIOPOLYMERS 79.1(2005):42264. |
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