Identification, expression, and purification of a unique stable domain from human HSPC144 protein
文献类型：期刊论文
| 作者 | Song, AX; Chang, YG; Gao, YG; Lin, XJ; Shi, YH; Lin, DH; Hang, QH; Hu, HY |
| 刊名 | PROTEIN EXPRESSION AND PURIFICATION
 |
| 出版日期 | 2005 |
| 卷号 | 42期号:1页码:146-152 |
| 关键词 | HSPC proteins DUF589 domain limited proteolysis expression purification NMR analysis |
| 通讯作者 | Hu, HY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Prote, Shanghai 200031, Peoples R China.,hyhu@sibs.ac.cn |
| 英文摘要 | HSPC144 is a newly identified gene in human CD34(+) hematopoietic stem/progenitor cells, In this work. we have expressed and purified the 225-residue protein from Escherichia coli BL21 (DE3) and identified a stable fragment HSPC144-P (residues 44 225) by limited proteolysis method. The HSPC144-P fragment exhibits high stability with a little increase of secondary structure percentage as compared with the full-length protein. We anticipated that the N-terminally truncated protein possesses a more compact structure. By sequence analysis, the proteolytic fragment shares a great similarity with DUF5899 domain. I previously identified domain with unknown function. This novel domain is highly conserved in Thy28 proteins and is worthy of structural and functional studies. We have subcloned this homologous domain from HSPC144 protein and purified to homogeneity for structure analysis. The N-15 and N-15/C-13-labeled DUF589 domain samples have been prepared successfully and determination of the NMR structure is in progress. (c) 2005 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | LIMITED PROTEOLYSIS ; SEQUENCE ; CLONING |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000229915500019 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1841]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Song, AX,Chang, YG,Gao, YG,et al. Identification, expression, and purification of a unique stable domain from human HSPC144 protein[J]. PROTEIN EXPRESSION AND PURIFICATION,2005,42(1):146-152. |
| APA | Song, AX.,Chang, YG.,Gao, YG.,Lin, XJ.,Shi, YH.,...&Hu, HY.(2005).Identification, expression, and purification of a unique stable domain from human HSPC144 protein.PROTEIN EXPRESSION AND PURIFICATION,42(1),146-152. |
| MLA | Song, AX,et al."Identification, expression, and purification of a unique stable domain from human HSPC144 protein".PROTEIN EXPRESSION AND PURIFICATION 42.1(2005):146-152. |
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