Antiapoptotic effect both in vivo and in vitro of A20 gene when transfected into rat hippocampal neurons
文献类型：期刊论文
| 作者 | Miao, HS; Yu, LY; Hui, GZ; Guo, LH |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2005 |
| 卷号 | 26期号:1页码:33-38 |
| 关键词 | A20 protein tumor necrosis factor primary hippocampal neurons middle cerebral artery infarction apoptosis |
| 通讯作者 | Guo, LH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,lhguo@sibs.ac.cn |
| 英文摘要 | Aim: To evaluate the antiapoptotic effect of the A20 gene in primary hippocampal neurons both in vivo and in vitro. Methods: Primary hippocampal neurons in embryonic day 18 (E 18) rats were transfected with the A20 gene by using the new Nucleofector electroporation transfection method. We then examined, whether A20-neurons possessed anti-apoptotic abilities after TNF-alpha stimulation in vitro. A20-neurons and pcDNA3-neurons were transplanted into the penumbra of the brains of rats that had been subjected to 90-min of ischemia induced by left middle cerebral artery occlusion (MCAO). Results: A20-neurons resisted TNF-alpha induced apoptosis in vitro. The apoptosis rate of neurons overexpressing A20 (28.46% +/- 3.87%) was lower than that in neurons transfected with pcDNA3 (53.06% +/- 5.36%). More A20-neurons survived in the penumbra both 3-d and 7-d after transplantation than did sham pcDNA3 neurons. Conclusion: The novel function of A20 may make it a potential targets for the gene therapy for neurological diseases. |
| 学科主题 | Chemistry; Pharmacology & Pharmacy |
| 类目[WOS] | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
| 关键词[WOS] | FOCAL CEREBRAL-ISCHEMIA ; NF-KAPPA-B ; ENDOTHELIAL-CELLS ; NECROSIS ; APOPTOSIS ; DISEASE ; ACTIVATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000226426600005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1845]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Miao, HS,Yu, LY,Hui, GZ,et al. Antiapoptotic effect both in vivo and in vitro of A20 gene when transfected into rat hippocampal neurons[J]. ACTA PHARMACOLOGICA SINICA,2005,26(1):33-38. |
| APA | Miao, HS,Yu, LY,Hui, GZ,&Guo, LH.(2005).Antiapoptotic effect both in vivo and in vitro of A20 gene when transfected into rat hippocampal neurons.ACTA PHARMACOLOGICA SINICA,26(1),33-38. |
| MLA | Miao, HS,et al."Antiapoptotic effect both in vivo and in vitro of A20 gene when transfected into rat hippocampal neurons".ACTA PHARMACOLOGICA SINICA 26.1(2005):33-38. |
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