Homologous up-regulation of androgen receptor expression by androgen in vascular smooth muscle cells
文献类型：期刊论文
| 作者 | Ma, R; Wu, SZ; Lin, QH |
| 刊名 | HORMONE RESEARCH
 |
| 出版日期 | 2005 |
| 卷号 | 63期号:1页码:42169 |
| 关键词 | androgen receptor testosterone auto-regulation of androgen receptor vascular smooth muscle cells androgen receptor transcriptional activity post-transcriptional regulation of androgen receptor |
| 通讯作者 | Wu, SZ (reprint author), Nanfang Hosp, Dept Cardiol, Guangzhou 510515, Peoples R China.,wusaizhu@fimmu.com |
| 英文摘要 | Objective: Androgens play an important role in the arterial vascular system, and androgen receptors (AR) have been identified in vascular smooth muscle cells (VSMCs). This study examined the effects of testosterone exposure on AR gene expression in cultured rat aortic smooth muscle cells. Methods: Changes in AR protein and messenger RNA ( mRNA) levels after androgen exposure were determined using immunoblotting and Northern blotting analysis respectively. Results: Treatment of synchronized VSMCs with testosterone increased both cytoplasmic and nuclear AR protein expression in a dose- and time-dependent fashion, whereas exposure of VSMCs to androgen for 10 min induced a transient down-regulation of AR protein. Meanwhile, AR mRNA level was also up-regulated, but to a much smaller extent. Pretreatment with transcription inhibitor and translation inhibitor repressed cytoplasmic AR protein levels to 46 and 12% ( means) of the androgen treatment control level respectively. Furthermore, androgen up-regulation of intracellular AR protein was partially inhibited by androgen antagonist. Conclusions: Androgen increases AR expression in VSMCs at the level of both transcription and non-transcription. Copyright (C) 2005 S. Karger AG, Basel. |
| 学科主题 | Endocrinology & Metabolism |
| 类目[WOS] | Endocrinology & Metabolism |
| 关键词[WOS] | CORONARY-HEART-DISEASE ; ANTIANDROGEN ACTION ; ESTROGEN-RECEPTOR ; DNA-SYNTHESIS ; SEX STEROIDS ; TESTOSTERONE ; ATHEROSCLEROSIS ; ARTERIES ; RELAXATION ; IMPAIRMENT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000226412400002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1847]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Ma, R,Wu, SZ,Lin, QH. Homologous up-regulation of androgen receptor expression by androgen in vascular smooth muscle cells[J]. HORMONE RESEARCH,2005,63(1):42169. |
| APA | Ma, R,Wu, SZ,&Lin, QH.(2005).Homologous up-regulation of androgen receptor expression by androgen in vascular smooth muscle cells.HORMONE RESEARCH,63(1),42169. |
| MLA | Ma, R,et al."Homologous up-regulation of androgen receptor expression by androgen in vascular smooth muscle cells".HORMONE RESEARCH 63.1(2005):42169. |
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