Effect of ribozyme against transforming growth factor beta 1 on biological character of activated HSCs
文献类型：期刊论文
| 作者 | Song, YH; Zhou, XM; Xue, XN; Liu, NZ; Tian, DA; Kong, XJ; Wu, XL; Lin, JS; Jin, YX |
| 刊名 | IUBMB LIFE
 |
| 出版日期 | 2005 |
| 卷号 | 57期号:1页码:31-39 |
| 关键词 | RNA catalytic transforming growth factor beta 1 hepatic stellate cell Character |
| 通讯作者 | Jin, YX (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,yxjin@sibs.ac.cn |
| 英文摘要 | Transforming growth factor beta 1 (TGF beta 1) is considered to be the principal contributor to liver fibrosis. So in this study the ribozymes against TGF beta 1 were designed. The in vitro cleavage activities of the ribozymes were assayed through incubation of (32)p- labeled target RNAs and (32)p- labeled ribozymes in different conditions. HSC-T6 cells were transfected with the eukaryotic constructs encoding ribozyme and disable ribozyme, then the stable cell clones were used to evaluate its antifibrotic characteristic through the effect of ribozyme on biological character of activated hepatic stellate cells (HSCs). The results demonstrated that two ribozymes (Rz803 and Rz1395) could cleave target RNAs into expected products effectively, Rz803 possessed better cleavage activity in vitro. Stable transfection of Rz803 into activated HSCs reduced TGF beta 1 expression in mRNA and protein level efficiently. The further studies demonstrated that Rz803 reduced deposition of collagen I, suppressed HSC proliferation, but had no effect on HSC activation in transfected HSC-T6 cells. Therefore, it indicated that Rz803 could reverse the character of activated HSCs by downregulating TGF beta 1 expression efficiently and diminishing TGF beta 1 signaling underlying activation of hepatic stellate cells. As the consequence, it would provide a potential therapeutic approach for liver fibrosis. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | HEPATIC STELLATE CELLS ; GROWTH-FACTOR-BETA ; LIVER FIBROSIS ; MESSENGER-RNA ; IN-VITRO ; TGF-BETA ; RAT ; RECEPTOR ; INHIBITION ; MICE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000228666000007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1848]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Song, YH,Zhou, XM,Xue, XN,et al. Effect of ribozyme against transforming growth factor beta 1 on biological character of activated HSCs[J]. IUBMB LIFE,2005,57(1):31-39. |
| APA | Song, YH.,Zhou, XM.,Xue, XN.,Liu, NZ.,Tian, DA.,...&Jin, YX.(2005).Effect of ribozyme against transforming growth factor beta 1 on biological character of activated HSCs.IUBMB LIFE,57(1),31-39. |
| MLA | Song, YH,et al."Effect of ribozyme against transforming growth factor beta 1 on biological character of activated HSCs".IUBMB LIFE 57.1(2005):31-39. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。