Identification, expression and functional characterization of the GRAL gene
文献类型：期刊论文
| 作者 | Li, ZH; Wang, BW; Wu, XF; Cheng, SY; Paraoan, L; Zhou, JW |
| 刊名 | JOURNAL OF NEUROCHEMISTRY
 |
| 出版日期 | 2005 |
| 卷号 | 95期号:2页码:361-376 |
| 关键词 | apoptosis glial cell-line-derived neurotrophic factor alpha receptor-like hippocampus PC12 |
| 通讯作者 | Zhou, JW (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Key Lab Proteom, Shanghai Inst Biol Sci, Bldg 23,Room 316,320 Yueyang Rd, Shanghai 200031, Peoples R China.,jwzhou@sibs.ac.cn |
| 英文摘要 | The glial cell line-derived neurotrophic factor ( GDNF) family is a group of neurotrophic factors with diverse biological functions. Members of the GDNF family exert their functions by interacting with a specific GDNF family receptor a (GFR alpha) and activation of the cRET. Here we report the identification and characterization of GDNF receptor-alpha-like (GRAL) gene. Sequence analysis indicated that GRAL is a distant homolog of the GFRa family, with 30% of its amino acid sequence identical to that of GFR alpha-3. There are two splice variants of GRAL: the full-length form (GRAL-A) represented by a 2080 bp mRNA and a short form (GRAL-B) represented by a 1833 bp mRNA. In adult mouse, GRAL transcripts have been found primarily in the CNS. In the developing mouse brain, the mRNA level of GRAL in the cerebrocortex and hippocampus reached a maximum at birth and declined afterwards. GRAL-A protein was localized predominantly in the plasma membrane. Overexpression of GRAL-A protected PC12 cells and cultured hippocampal neurons from serum starvation-induced cell apoptosis. The neuroprotective effect of GRAL was associated with marked inhibition of the Jun-N-terminal kinase signaling pathway. Our results suggest that GRAL belongs to a superfamily of GFR alpha and might take part in neuroprotection and brain development. |
| 学科主题 | Biochemistry & Molecular Biology; Neurosciences & Neurology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Neurosciences |
| 关键词[WOS] | NEUROTROPHIC FACTOR FAMILY ; NERVE GROWTH-FACTOR ; PC12 CELLS ; RECEPTOR COMPLEX ; TYROSINE KINASE ; GDNF FAMILY ; PERSEPHIN ; ACTIVATION ; NEURTURIN ; SURVIVAL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000232142900007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/1855]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, ZH,Wang, BW,Wu, XF,et al. Identification, expression and functional characterization of the GRAL gene[J]. JOURNAL OF NEUROCHEMISTRY,2005,95(2):361-376. |
| APA | Li, ZH,Wang, BW,Wu, XF,Cheng, SY,Paraoan, L,&Zhou, JW.(2005).Identification, expression and functional characterization of the GRAL gene.JOURNAL OF NEUROCHEMISTRY,95(2),361-376. |
| MLA | Li, ZH,et al."Identification, expression and functional characterization of the GRAL gene".JOURNAL OF NEUROCHEMISTRY 95.2(2005):361-376. |
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